Expression patterns of five polymorphic membrane proteins during the Chlamydia abortus developmental cycle

Nick M. Wheelhouse, Michelle Sait, Kim Wilson, Kevin Aitchison, Kevin McLean, David George Emslie Smith, David Longbottom

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    It has been suggested that polymorphic membrane proteins (Pmps) belonging to the Type V autotransporter protein family play an important role in the pathogenesis of Chlamydia abortus (C. abortus; formerly Chlamydophila abortus) infection. In a previous study we demonstrated the expression of all the pmps at the transcriptional level. The purpose of this study was to measure the number of Pmp positive inclusions throughout the C. abortus developmental cycle to investigate heterogeneity in expression patterns. McCoy cells were infected with C. abortus and analysed for Pmp expression over a 72 h period by fluorescent immunocytochemistry. Pmp18D could be detected at all analysed time points, and could only be accurately quantified from 36 hpi while Pmp10G positive inclusions could be visualised from 36hpi. Expression of Pmps 13G, 16G and 17G could only be visualised later in the cycle and within less than half of visualised inclusions. These results indicate that while expression of specific Pmps is constitutive (Pmp18D), the pattern of expression of other Pmps is more variable. This suggests that different members of the Pmp family may play different roles within the developmental cycle of the organism, with some (Pmps10G and 18D) having roles throughout the cycle, while the heterogeneity of expression of others may aid in antigenic variation.

    Original languageEnglish
    Pages (from-to)525-529
    Number of pages5
    JournalVeterinary Microbiology
    Volume160
    Issue number3-4
    DOIs
    Publication statusPublished - 7 Dec 2012

    Keywords

    • Animals
    • Bacterial Outer Membrane Proteins
    • Cell Line
    • Chlamydia
    • Chlamydia Infections
    • Gene Expression Profiling
    • Gene Expression Regulation, Bacterial
    • Mice

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