Expression by Lawsonia intracellularis of type III secretion system components during infection

M. Pilar Alberdi, Eleanor Watson, Gina E. M. McAllister, Jennifer D. Harris, Edith A. Paxton, Jill R. Thomson, David George Emslie Smith

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    Abstract

    Contact-dependent secretion systems, such as the type III secretion system (T3SS), have been shown to play significant roles in the pathogenicity of many gram-negative bacterial pathogens. Lawsonia intracellularis is a novel, obligate intracellular gram-negative bacterium, which has been identified as the etiological agent of proliferative enteropathies in numerous animal species. Analysis of the genome sequence of the L. intracellularis strain PHE/MN1-00 has revealed the presence of a T3SS secretion system in this bacterium. In this study we aimed to determine whether this important virulence mechanism is also present in L. intracellularis strain LR189/5/83. Using a PCR-based approach, we verified the presence of a genomic region encoding a T3SS. Specifically, a gene highly homologous to the yscN energiser component of the prototypic T3SS of Yersinia spp. was identified and termed lscN. Two further open reading frames (ORFs) contiguous with lscN were also identified: lscO and lscQ, which are also homologues of ORFs within the T3SS of Yersinia spp. To establish whether this T3SS may be functional, expression was monitored directly by RT-PCR and indirectly by detection of serological responses in vaccinated and infected animals. Transcripts for lscN and lscQ were detected and purified rLscQ was recognized by antiserum from infected pigs, indicating expression in vivo during infection. By analogy to other bacteria, this T3SS may be crucial for intracellular development and is likely to play a significant role in the virulence of this unusual pathogen.

    Original languageEnglish
    Pages (from-to)298-303
    Number of pages6
    JournalVeterinary Microbiology
    Volume139
    Issue number3-4
    DOIs
    Publication statusPublished - 18 Nov 2009

    Keywords

    • Amino Acid Sequence
    • Animals
    • Bacterial Proteins
    • DNA, Bacterial
    • Gene Expression Regulation, Bacterial
    • Lawsonia Bacteria
    • Molecular Sequence Data
    • RNA, Bacterial
    • Reverse Transcriptase Polymerase Chain Reaction
    • Sequence Alignment
    • Swine
    • Virulence

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  • Cite this

    Alberdi, M. P., Watson, E., McAllister, G. E. M., Harris, J. D., Paxton, E. A., Thomson, J. R., & Smith, D. G. E. (2009). Expression by Lawsonia intracellularis of type III secretion system components during infection. Veterinary Microbiology, 139(3-4), 298-303. https://doi.org/10.1016/j.vetmic.2009.06.022