Examining solvent/fermentation broth interfaces

E. Doherty, J. A S Goodwin, J. C. Slaughter, L. R. Weatherley

Research output: Contribution to journalArticle

Abstract

A microscopic method was developed to allow the visual investigation of cells at solvent/fermentation broth interfaces, using glass capillary tubes of set dimensions. This method allows the interfacial distribution of cells to be investigated and photographed. Fermentation broths of a dimorphic micro-organism Aureobasidium pullulans (which can exist in both a filamentous and yeast-like form), and the solvent n-decanol were chosen for this study. It was found that cell behaviour was dependent on the cell morphology, with the filamentous cells gathering outside the viewing range of the liquid/liquid interface. The yeast-like cells were randomly distributed throughout the broth, and visible in the area of the interface. The cells did not form a continuous layer at the interface, as hypothesised by Crabbe.

Original languageEnglish
Pages (from-to)85-88
Number of pages4
JournalBioseparation
Volume4
Issue number2
Publication statusPublished - Apr 1994

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    Doherty, E., Goodwin, J. A. S., Slaughter, J. C., & Weatherley, L. R. (1994). Examining solvent/fermentation broth interfaces. Bioseparation, 4(2), 85-88.