Evidence for phosphonate usage in the coral holobiont

S. Thomas, Heidi Burdett, B. Temperton, R. Wick, D. Snelling, J. W. McGrath, J. P. Quinn, C. Munn, J. A. Gilbert

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20 Citations (Scopus)

Abstract

Phosphonates are characterized by a stable carbon-phosphorus bond and commonly occur as lipid conjugates in invertebrate cell membranes. Phosphonoacetate hydrolase encoded by the phnA gene, catalyses the cleavage of phosphonoacetate to acetate and phosphate. In this study, we demonstrate the unusually high phnA diversity in coral-associated bacteria. The holobiont of eight coral species tested positive when screened for phnA using degenerate primers. In two soft coral species, Sinularia and Discosoma, sequencing of the phnA gene showed 13 distinct groups on the basis of 90% sequence identity across 100% of the sequence. A total of 16 bacterial taxa capable of using phosphonoacetate as the sole carbon and phosphorus source were isolated; 8 of which had a phnA+ genotype. This study enhances our understanding of the wide taxonomic and environmental distribution of phnA, and highlights the importance of phosphonates in marine ecosystems.

Original languageEnglish
Pages (from-to)459-461
Number of pages3
JournalISME Journal
Volume4
Issue number3
DOIs
Publication statusPublished - Mar 2010

Keywords

  • Alkaline Phosphatase
  • Animals
  • Anthozoa
  • Bacteria
  • Bacterial Proteins
  • Biodiversity
  • DNA, Bacterial
  • DNA, Ribosomal
  • Molecular Sequence Data
  • Organophosphonates
  • Phosphoric Monoester Hydrolases
  • RNA, Ribosomal, 16S
  • Sequence Analysis, DNA
  • Journal Article
  • Research Support, Non-U.S. Gov't

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  • Cite this

    Thomas, S., Burdett, H., Temperton, B., Wick, R., Snelling, D., McGrath, J. W., Quinn, J. P., Munn, C., & Gilbert, J. A. (2010). Evidence for phosphonate usage in the coral holobiont. ISME Journal, 4(3), 459-461. https://doi.org/10.1038/ismej.2009.129