Enzyme immobilisation using siliceous mesoporous molecular sieves

Humphrey Hak Ping Yiu, Paul A Wright, Nigel P Botting

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198 Citations (Scopus)

Abstract

The use of mesoporous molecular sieves in enzyme immobilisation has been studied. Three different types of mesoporous sieves (MCM-41, MCM-48 and SBA-15) were selected because of the differences in their pore dimensions and structures. Commercially available porous silica gel was chosen for comparison. The model enzyme chosen in this study was trypsin. The samples of immobilised trypsin were active For the hydrolysis of N-alpha -benzoyl-DL-arginine-4-nitro-anilide (BAPNA). The amount of enzyme adsorbed on the supports was found to be related to the pore size of the molecular sieves. Moreover. the pore size and the structure of the support also affected the activity of the supported enzymes. (C) 2001 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)763-768
Number of pages6
JournalMicroporous and Mesoporous Materials
Volume44-45
DOIs
Publication statusPublished - 6 Apr 2001

Keywords

  • enzyme immobilisation
  • trypsin
  • mesoporous molecular sieves
  • SBA-15
  • MCM-41
  • CATALYSIS

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