Enzyme immobilisation using siliceous mesoporous molecular sieves

Humphrey Hak Ping Yiu; Paul A Wright; Nigel P Botting

doi:10.1016/S1387-1811(01)00258-X

Enzyme immobilisation using siliceous mesoporous molecular sieves

Humphrey Hak Ping Yiu, Paul A Wright, Nigel P Botting

School of Engineering & Physical Sciences

Research output: Contribution to journal › Article › peer-review

206 Citations (Scopus)

Abstract

The use of mesoporous molecular sieves in enzyme immobilisation has been studied. Three different types of mesoporous sieves (MCM-41, MCM-48 and SBA-15) were selected because of the differences in their pore dimensions and structures. Commercially available porous silica gel was chosen for comparison. The model enzyme chosen in this study was trypsin. The samples of immobilised trypsin were active For the hydrolysis of N-alpha -benzoyl-DL-arginine-4-nitro-anilide (BAPNA). The amount of enzyme adsorbed on the supports was found to be related to the pore size of the molecular sieves. Moreover. the pore size and the structure of the support also affected the activity of the supported enzymes. (C) 2001 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	763-768
Number of pages	6
Journal	Microporous and Mesoporous Materials
Volume	44-45
DOIs	https://doi.org/10.1016/S1387-1811(01)00258-X
Publication status	Published - 6 Apr 2001

Keywords

enzyme immobilisation
trypsin
mesoporous molecular sieves
SBA-15
MCM-41
CATALYSIS

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10.1016/S1387-1811(01)00258-X

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title = "Enzyme immobilisation using siliceous mesoporous molecular sieves",

abstract = "The use of mesoporous molecular sieves in enzyme immobilisation has been studied. Three different types of mesoporous sieves (MCM-41, MCM-48 and SBA-15) were selected because of the differences in their pore dimensions and structures. Commercially available porous silica gel was chosen for comparison. The model enzyme chosen in this study was trypsin. The samples of immobilised trypsin were active For the hydrolysis of N-alpha -benzoyl-DL-arginine-4-nitro-anilide (BAPNA). The amount of enzyme adsorbed on the supports was found to be related to the pore size of the molecular sieves. Moreover. the pore size and the structure of the support also affected the activity of the supported enzymes. (C) 2001 Elsevier Science B.V. All rights reserved.",

keywords = "enzyme immobilisation, trypsin, mesoporous molecular sieves, SBA-15, MCM-41, CATALYSIS",

author = "Yiu, {Humphrey Hak Ping} and Wright, {Paul A} and Botting, {Nigel P}",

year = "2001",

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doi = "10.1016/S1387-1811(01)00258-X",

language = "English",

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TY - JOUR

T1 - Enzyme immobilisation using siliceous mesoporous molecular sieves

AU - Yiu, Humphrey Hak Ping

AU - Wright, Paul A

AU - Botting, Nigel P

PY - 2001/4/6

Y1 - 2001/4/6

N2 - The use of mesoporous molecular sieves in enzyme immobilisation has been studied. Three different types of mesoporous sieves (MCM-41, MCM-48 and SBA-15) were selected because of the differences in their pore dimensions and structures. Commercially available porous silica gel was chosen for comparison. The model enzyme chosen in this study was trypsin. The samples of immobilised trypsin were active For the hydrolysis of N-alpha -benzoyl-DL-arginine-4-nitro-anilide (BAPNA). The amount of enzyme adsorbed on the supports was found to be related to the pore size of the molecular sieves. Moreover. the pore size and the structure of the support also affected the activity of the supported enzymes. (C) 2001 Elsevier Science B.V. All rights reserved.

AB - The use of mesoporous molecular sieves in enzyme immobilisation has been studied. Three different types of mesoporous sieves (MCM-41, MCM-48 and SBA-15) were selected because of the differences in their pore dimensions and structures. Commercially available porous silica gel was chosen for comparison. The model enzyme chosen in this study was trypsin. The samples of immobilised trypsin were active For the hydrolysis of N-alpha -benzoyl-DL-arginine-4-nitro-anilide (BAPNA). The amount of enzyme adsorbed on the supports was found to be related to the pore size of the molecular sieves. Moreover. the pore size and the structure of the support also affected the activity of the supported enzymes. (C) 2001 Elsevier Science B.V. All rights reserved.

KW - enzyme immobilisation

KW - trypsin

KW - mesoporous molecular sieves

KW - SBA-15

KW - MCM-41

KW - CATALYSIS

U2 - 10.1016/S1387-1811(01)00258-X

DO - 10.1016/S1387-1811(01)00258-X

M3 - Article

SN - 1387-1811

VL - 44-45

SP - 763

EP - 768

JO - Microporous and Mesoporous Materials

JF - Microporous and Mesoporous Materials

ER -

Enzyme immobilisation using siliceous mesoporous molecular sieves

Abstract

Keywords

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