Enhancement of biomolecule transport by electroporation: A review of theory and practical application to transformation of Corynebacterium glutamicum

Theodora Tryfona, Mark T. Bustard

Research output: Contribution to journalLiterature review

32 Citations (Scopus)

Abstract

Selective and reversible permeabilization of the cell wall permeability barrier is the focus for many biotechnological applications. In this article, the basic principles for reversible membrane permeabilization, based on biological, chemical, and physical methods are reviewed. Emphasis is given to electroporation (electropermeabilization) which tends to be the most popular method for membrane permeabilization and for introduction of foreign molecules into the cells. The applications of this method in industrial processes as well as the critical factors and parameters which affect the success of this approach are discussed. The different strategies developed throughout the years for increased transformation efficiencies of the industrially important amino acid-overproducing bacterium Corynebacterium glutamicum, are also summarized. © 2005 Wiley Periodicals, Inc.

Original languageEnglish
Pages (from-to)413-423
Number of pages11
JournalBiotechnology and Bioengineering
Volume93
Issue number3
DOIs
Publication statusPublished - 20 Feb 2006

Keywords

  • Corynebacterium glutamicum
  • Electroporation
  • Electrotransformation
  • Membrane permeabilization

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