Endoscopic sensing of pH in the distal lung (Conference Presentation)

Debaditya Choudhury, Michael George Tanner, Sarah McAughtrie, Fei Yu, Bethany Mills, Tushar R. Choudhary, Sohan Seth, Thomas Craven, James M. Stone, Ioulia K. Mati, Colin J. Campbell, Mark Bradley, Christopher K. I. Williams, Kevin Dhaliwal, Timothy A. Birks, Robert R. Thomson

Research output: Contribution to conferenceOther

Abstract

In healthy humans, the physiological state in the distal lung alveolar acinar units is tightly regulated by normal homeostatic mechanisms. Pulmonary abnormalities such as chronic obstructive pulmonary disease, that are characterized by recurrent cycles of inflammation and infection involving dense infiltration by myeloid derived peripheral blood cells, may result in significant perturbation of the homeostatic baselines of physiology in addition to host tissue damage. Therefore, the ability to quantify and monitor physiology (e.g. pH, glucose level, oxygen tension) within the alveolar acinar units would provide a key biomarker of distal lung innate defence. Although in vitro modeling of fundamental biological processes show remarkable sensitivity to physiological aberrations, little is known about the physiological state of the distal lung due to the inability to concurrently access the alveolar sacs and perform real-time sensing. Here we report on previously unobtainable measurements of alveolar pH using a fiber-optic optrode and surface enhanced Raman spectroscopy (SERS) and show that alveolar pH changes in response to ventilation. The endoscope-deployable optrode consisted of para-mercaptobenzoic acid functionalized 150 nm gold nanoshells located at the distal end, and an asymmetric dual-core optical fiber designed for spatially separated optical pump delivery and SERS signal collection in order to circumvent the unwanted Raman signal originating from the fiber itself. We demonstrate a ~ 100-fold increase in SERS signal-to-fiber background ratio and pH sensing at multiple sites in the respiratory acinar units of a whole ex vivo ovine lung model with a measurement accuracy of ± 0.07 pH units.
Original languageEnglish
Pages100410B
DOIs
Publication statusPublished - 19 Apr 2017
EventSPIE BiOS 2017 - San Fransisco, United States
Duration: 28 Jan 20172 Feb 2017

Conference

ConferenceSPIE BiOS 2017
CountryUnited States
CitySan Fransisco
Period28/01/172/02/17

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Raman Spectrum Analysis
Lung
Nanoshells
Biological Phenomena
Optical Fibers
Endoscopes
Gold
Chronic Obstructive Pulmonary Disease
Ventilation
Blood Cells
Sheep
Biomarkers
Oxygen
Inflammation
Glucose
Acids
Infection

Cite this

Choudhury, D., Tanner, M. G., McAughtrie, S., Yu, F., Mills, B., Choudhary, T. R., ... Thomson, R. R. (2017). Endoscopic sensing of pH in the distal lung (Conference Presentation). 100410B. SPIE BiOS 2017, San Fransisco, United States. https://doi.org/10.1117/12.2251925
Choudhury, Debaditya ; Tanner, Michael George ; McAughtrie, Sarah ; Yu, Fei ; Mills, Bethany ; Choudhary, Tushar R. ; Seth, Sohan ; Craven, Thomas ; Stone, James M. ; Mati, Ioulia K. ; Campbell, Colin J. ; Bradley, Mark ; Williams, Christopher K. I. ; Dhaliwal, Kevin ; Birks, Timothy A. ; Thomson, Robert R. / Endoscopic sensing of pH in the distal lung (Conference Presentation). SPIE BiOS 2017, San Fransisco, United States.
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title = "Endoscopic sensing of pH in the distal lung (Conference Presentation)",
abstract = "In healthy humans, the physiological state in the distal lung alveolar acinar units is tightly regulated by normal homeostatic mechanisms. Pulmonary abnormalities such as chronic obstructive pulmonary disease, that are characterized by recurrent cycles of inflammation and infection involving dense infiltration by myeloid derived peripheral blood cells, may result in significant perturbation of the homeostatic baselines of physiology in addition to host tissue damage. Therefore, the ability to quantify and monitor physiology (e.g. pH, glucose level, oxygen tension) within the alveolar acinar units would provide a key biomarker of distal lung innate defence. Although in vitro modeling of fundamental biological processes show remarkable sensitivity to physiological aberrations, little is known about the physiological state of the distal lung due to the inability to concurrently access the alveolar sacs and perform real-time sensing. Here we report on previously unobtainable measurements of alveolar pH using a fiber-optic optrode and surface enhanced Raman spectroscopy (SERS) and show that alveolar pH changes in response to ventilation. The endoscope-deployable optrode consisted of para-mercaptobenzoic acid functionalized 150 nm gold nanoshells located at the distal end, and an asymmetric dual-core optical fiber designed for spatially separated optical pump delivery and SERS signal collection in order to circumvent the unwanted Raman signal originating from the fiber itself. We demonstrate a ~ 100-fold increase in SERS signal-to-fiber background ratio and pH sensing at multiple sites in the respiratory acinar units of a whole ex vivo ovine lung model with a measurement accuracy of ± 0.07 pH units.",
author = "Debaditya Choudhury and Tanner, {Michael George} and Sarah McAughtrie and Fei Yu and Bethany Mills and Choudhary, {Tushar R.} and Sohan Seth and Thomas Craven and Stone, {James M.} and Mati, {Ioulia K.} and Campbell, {Colin J.} and Mark Bradley and Williams, {Christopher K. I.} and Kevin Dhaliwal and Birks, {Timothy A.} and Thomson, {Robert R.}",
note = "Presentation only; SPIE BiOS 2017 ; Conference date: 28-01-2017 Through 02-02-2017",
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Choudhury, D, Tanner, MG, McAughtrie, S, Yu, F, Mills, B, Choudhary, TR, Seth, S, Craven, T, Stone, JM, Mati, IK, Campbell, CJ, Bradley, M, Williams, CKI, Dhaliwal, K, Birks, TA & Thomson, RR 2017, 'Endoscopic sensing of pH in the distal lung (Conference Presentation)' SPIE BiOS 2017, San Fransisco, United States, 28/01/17 - 2/02/17, pp. 100410B. https://doi.org/10.1117/12.2251925

Endoscopic sensing of pH in the distal lung (Conference Presentation). / Choudhury, Debaditya; Tanner, Michael George; McAughtrie, Sarah; Yu, Fei; Mills, Bethany; Choudhary, Tushar R.; Seth, Sohan; Craven, Thomas; Stone, James M.; Mati, Ioulia K.; Campbell, Colin J.; Bradley, Mark; Williams, Christopher K. I.; Dhaliwal, Kevin; Birks, Timothy A.; Thomson, Robert R.

2017. 100410B SPIE BiOS 2017, San Fransisco, United States.

Research output: Contribution to conferenceOther

TY - CONF

T1 - Endoscopic sensing of pH in the distal lung (Conference Presentation)

AU - Choudhury, Debaditya

AU - Tanner, Michael George

AU - McAughtrie, Sarah

AU - Yu, Fei

AU - Mills, Bethany

AU - Choudhary, Tushar R.

AU - Seth, Sohan

AU - Craven, Thomas

AU - Stone, James M.

AU - Mati, Ioulia K.

AU - Campbell, Colin J.

AU - Bradley, Mark

AU - Williams, Christopher K. I.

AU - Dhaliwal, Kevin

AU - Birks, Timothy A.

AU - Thomson, Robert R.

N1 - Presentation only

PY - 2017/4/19

Y1 - 2017/4/19

N2 - In healthy humans, the physiological state in the distal lung alveolar acinar units is tightly regulated by normal homeostatic mechanisms. Pulmonary abnormalities such as chronic obstructive pulmonary disease, that are characterized by recurrent cycles of inflammation and infection involving dense infiltration by myeloid derived peripheral blood cells, may result in significant perturbation of the homeostatic baselines of physiology in addition to host tissue damage. Therefore, the ability to quantify and monitor physiology (e.g. pH, glucose level, oxygen tension) within the alveolar acinar units would provide a key biomarker of distal lung innate defence. Although in vitro modeling of fundamental biological processes show remarkable sensitivity to physiological aberrations, little is known about the physiological state of the distal lung due to the inability to concurrently access the alveolar sacs and perform real-time sensing. Here we report on previously unobtainable measurements of alveolar pH using a fiber-optic optrode and surface enhanced Raman spectroscopy (SERS) and show that alveolar pH changes in response to ventilation. The endoscope-deployable optrode consisted of para-mercaptobenzoic acid functionalized 150 nm gold nanoshells located at the distal end, and an asymmetric dual-core optical fiber designed for spatially separated optical pump delivery and SERS signal collection in order to circumvent the unwanted Raman signal originating from the fiber itself. We demonstrate a ~ 100-fold increase in SERS signal-to-fiber background ratio and pH sensing at multiple sites in the respiratory acinar units of a whole ex vivo ovine lung model with a measurement accuracy of ± 0.07 pH units.

AB - In healthy humans, the physiological state in the distal lung alveolar acinar units is tightly regulated by normal homeostatic mechanisms. Pulmonary abnormalities such as chronic obstructive pulmonary disease, that are characterized by recurrent cycles of inflammation and infection involving dense infiltration by myeloid derived peripheral blood cells, may result in significant perturbation of the homeostatic baselines of physiology in addition to host tissue damage. Therefore, the ability to quantify and monitor physiology (e.g. pH, glucose level, oxygen tension) within the alveolar acinar units would provide a key biomarker of distal lung innate defence. Although in vitro modeling of fundamental biological processes show remarkable sensitivity to physiological aberrations, little is known about the physiological state of the distal lung due to the inability to concurrently access the alveolar sacs and perform real-time sensing. Here we report on previously unobtainable measurements of alveolar pH using a fiber-optic optrode and surface enhanced Raman spectroscopy (SERS) and show that alveolar pH changes in response to ventilation. The endoscope-deployable optrode consisted of para-mercaptobenzoic acid functionalized 150 nm gold nanoshells located at the distal end, and an asymmetric dual-core optical fiber designed for spatially separated optical pump delivery and SERS signal collection in order to circumvent the unwanted Raman signal originating from the fiber itself. We demonstrate a ~ 100-fold increase in SERS signal-to-fiber background ratio and pH sensing at multiple sites in the respiratory acinar units of a whole ex vivo ovine lung model with a measurement accuracy of ± 0.07 pH units.

U2 - 10.1117/12.2251925

DO - 10.1117/12.2251925

M3 - Other

SP - 100410B

ER -

Choudhury D, Tanner MG, McAughtrie S, Yu F, Mills B, Choudhary TR et al. Endoscopic sensing of pH in the distal lung (Conference Presentation). 2017. SPIE BiOS 2017, San Fransisco, United States. https://doi.org/10.1117/12.2251925