Electrically enhanced enzymic hydrolysis of vegetable oils using lipase from Candida rugosa

J. C. Slaughter, L. R. Weatherley, A. Wilkinson

Research output: Contribution to journalArticle

Abstract

Conditions have been established for the microdispersion of aqueous solutions of a lipase from Candida rugosa in columns of vegetable oil using a high electrical field. In a single pass through the column the enzyme solution lost just over 10% of its activity. Operation of the column over 8 h with continuous pumping of the enzyme effected over 80% hydrolysis of the oil without breakdown of the microdispersion or formation of an enzyme/oil emulsion. Activity of the enzyme was dependent on a negative charge being applied to the central electrode of the apparatus. Both the degree of hydrolysis achieved and the activity of recovered enzyme were superior to that from a simple stirred reactor of the same scale. In addition, the electrically enhanced system absorbed less than 40% of the energy demanded by the stirred system.

Original languageEnglish
Pages (from-to)293-296
Number of pages4
JournalEnzyme and Microbial Technology
Volume15
Issue number4
DOIs
Publication statusPublished - Apr 1993

Keywords

  • electrically enhanced
  • fatty acids
  • high voltage
  • hydrolysis
  • lipase
  • oil

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