Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout

Tessa M. Scown, Eduarda M. Santos, Blair D. Johnston, Birgit Gaiser, Mohammed Baalousha, Svetlin Mitov, Jamie R. Lead, Vicki Stone, Teresa F. Fernandes, Mark Jepson, Ronny van Aerle, Charles R. Tyler

    Research output: Contribution to journalArticle

    Abstract

    Despite increasing application of silver nanoparticles (NPs) in industry and consumer products, there is still little known about their potential toxicity, particularly to organisms in aquatic environments. To investigate the fate and effects of silver NPs in fish, rainbow trout (Oncorhynchus mykiss) were exposed via the water to commercial silver particles of three nominal sizes: 10 nm (N-10), 35 nm (N-35), and 600-1600 nm (N-Bulk), and to silver nitrate for 10 days. Uptake into the gills, liver, and kidneys was quantified by inductively coupled plasma-optical emission spectrometry, and levels of lipid peroxidation in gills, liver, and blood were determined by measurements of thiobarbituric acid reactive substances. Expression of a suite of genes, namely cyp1a2, cyp3a45, hsp70a, gpx, and g6pd, known to be involved in a range of toxicological response to xenobiotics was analyzed in the gills and liver using real-time PCR. Uptake of silver particles from the water into the tissues of exposed fish was low but nevertheless occurred for current estimated environmental exposures. Of the silver particles tested, N-10 were found to be the most highly concentrated within gill tissues and N-10 and N-Bulk were the most highly concentrated in liver. There were no effects on lipid peroxidation in any of the tissues analyzed for any of the silver particles tested, and this is likely due to the low uptake rates. However, exposure to N-10 particles was found to induce expression of cyp1a2 in the gills, suggesting a possible increase in oxidative metabolism in this tissue.

    Original languageEnglish
    Pages (from-to)521-534
    Number of pages14
    JournalToxicological Sciences
    Volume115
    Issue number2
    DOIs
    Publication statusPublished - Jun 2010

    Cite this

    Scown, T. M., Santos, E. M., Johnston, B. D., Gaiser, B., Baalousha, M., Mitov, S., ... Tyler, C. R. (2010). Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout. Toxicological Sciences , 115(2), 521-534. https://doi.org/10.1093/toxsci/kfq076
    Scown, Tessa M. ; Santos, Eduarda M. ; Johnston, Blair D. ; Gaiser, Birgit ; Baalousha, Mohammed ; Mitov, Svetlin ; Lead, Jamie R. ; Stone, Vicki ; Fernandes, Teresa F. ; Jepson, Mark ; van Aerle, Ronny ; Tyler, Charles R. / Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout. In: Toxicological Sciences . 2010 ; Vol. 115, No. 2. pp. 521-534.
    @article{63469b8511fb4a3d9d651e9cf91adefe,
    title = "Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout",
    abstract = "Despite increasing application of silver nanoparticles (NPs) in industry and consumer products, there is still little known about their potential toxicity, particularly to organisms in aquatic environments. To investigate the fate and effects of silver NPs in fish, rainbow trout (Oncorhynchus mykiss) were exposed via the water to commercial silver particles of three nominal sizes: 10 nm (N-10), 35 nm (N-35), and 600-1600 nm (N-Bulk), and to silver nitrate for 10 days. Uptake into the gills, liver, and kidneys was quantified by inductively coupled plasma-optical emission spectrometry, and levels of lipid peroxidation in gills, liver, and blood were determined by measurements of thiobarbituric acid reactive substances. Expression of a suite of genes, namely cyp1a2, cyp3a45, hsp70a, gpx, and g6pd, known to be involved in a range of toxicological response to xenobiotics was analyzed in the gills and liver using real-time PCR. Uptake of silver particles from the water into the tissues of exposed fish was low but nevertheless occurred for current estimated environmental exposures. Of the silver particles tested, N-10 were found to be the most highly concentrated within gill tissues and N-10 and N-Bulk were the most highly concentrated in liver. There were no effects on lipid peroxidation in any of the tissues analyzed for any of the silver particles tested, and this is likely due to the low uptake rates. However, exposure to N-10 particles was found to induce expression of cyp1a2 in the gills, suggesting a possible increase in oxidative metabolism in this tissue.",
    author = "Scown, {Tessa M.} and Santos, {Eduarda M.} and Johnston, {Blair D.} and Birgit Gaiser and Mohammed Baalousha and Svetlin Mitov and Lead, {Jamie R.} and Vicki Stone and Fernandes, {Teresa F.} and Mark Jepson and {van Aerle}, Ronny and Tyler, {Charles R.}",
    year = "2010",
    month = "6",
    doi = "10.1093/toxsci/kfq076",
    language = "English",
    volume = "115",
    pages = "521--534",
    journal = "Toxicological Sciences",
    issn = "1096-6080",
    publisher = "Oxford University Press",
    number = "2",

    }

    Scown, TM, Santos, EM, Johnston, BD, Gaiser, B, Baalousha, M, Mitov, S, Lead, JR, Stone, V, Fernandes, TF, Jepson, M, van Aerle, R & Tyler, CR 2010, 'Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout', Toxicological Sciences , vol. 115, no. 2, pp. 521-534. https://doi.org/10.1093/toxsci/kfq076

    Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout. / Scown, Tessa M.; Santos, Eduarda M.; Johnston, Blair D.; Gaiser, Birgit; Baalousha, Mohammed; Mitov, Svetlin; Lead, Jamie R.; Stone, Vicki; Fernandes, Teresa F.; Jepson, Mark; van Aerle, Ronny; Tyler, Charles R.

    In: Toxicological Sciences , Vol. 115, No. 2, 06.2010, p. 521-534.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout

    AU - Scown, Tessa M.

    AU - Santos, Eduarda M.

    AU - Johnston, Blair D.

    AU - Gaiser, Birgit

    AU - Baalousha, Mohammed

    AU - Mitov, Svetlin

    AU - Lead, Jamie R.

    AU - Stone, Vicki

    AU - Fernandes, Teresa F.

    AU - Jepson, Mark

    AU - van Aerle, Ronny

    AU - Tyler, Charles R.

    PY - 2010/6

    Y1 - 2010/6

    N2 - Despite increasing application of silver nanoparticles (NPs) in industry and consumer products, there is still little known about their potential toxicity, particularly to organisms in aquatic environments. To investigate the fate and effects of silver NPs in fish, rainbow trout (Oncorhynchus mykiss) were exposed via the water to commercial silver particles of three nominal sizes: 10 nm (N-10), 35 nm (N-35), and 600-1600 nm (N-Bulk), and to silver nitrate for 10 days. Uptake into the gills, liver, and kidneys was quantified by inductively coupled plasma-optical emission spectrometry, and levels of lipid peroxidation in gills, liver, and blood were determined by measurements of thiobarbituric acid reactive substances. Expression of a suite of genes, namely cyp1a2, cyp3a45, hsp70a, gpx, and g6pd, known to be involved in a range of toxicological response to xenobiotics was analyzed in the gills and liver using real-time PCR. Uptake of silver particles from the water into the tissues of exposed fish was low but nevertheless occurred for current estimated environmental exposures. Of the silver particles tested, N-10 were found to be the most highly concentrated within gill tissues and N-10 and N-Bulk were the most highly concentrated in liver. There were no effects on lipid peroxidation in any of the tissues analyzed for any of the silver particles tested, and this is likely due to the low uptake rates. However, exposure to N-10 particles was found to induce expression of cyp1a2 in the gills, suggesting a possible increase in oxidative metabolism in this tissue.

    AB - Despite increasing application of silver nanoparticles (NPs) in industry and consumer products, there is still little known about their potential toxicity, particularly to organisms in aquatic environments. To investigate the fate and effects of silver NPs in fish, rainbow trout (Oncorhynchus mykiss) were exposed via the water to commercial silver particles of three nominal sizes: 10 nm (N-10), 35 nm (N-35), and 600-1600 nm (N-Bulk), and to silver nitrate for 10 days. Uptake into the gills, liver, and kidneys was quantified by inductively coupled plasma-optical emission spectrometry, and levels of lipid peroxidation in gills, liver, and blood were determined by measurements of thiobarbituric acid reactive substances. Expression of a suite of genes, namely cyp1a2, cyp3a45, hsp70a, gpx, and g6pd, known to be involved in a range of toxicological response to xenobiotics was analyzed in the gills and liver using real-time PCR. Uptake of silver particles from the water into the tissues of exposed fish was low but nevertheless occurred for current estimated environmental exposures. Of the silver particles tested, N-10 were found to be the most highly concentrated within gill tissues and N-10 and N-Bulk were the most highly concentrated in liver. There were no effects on lipid peroxidation in any of the tissues analyzed for any of the silver particles tested, and this is likely due to the low uptake rates. However, exposure to N-10 particles was found to induce expression of cyp1a2 in the gills, suggesting a possible increase in oxidative metabolism in this tissue.

    U2 - 10.1093/toxsci/kfq076

    DO - 10.1093/toxsci/kfq076

    M3 - Article

    C2 - 20219766

    VL - 115

    SP - 521

    EP - 534

    JO - Toxicological Sciences

    JF - Toxicological Sciences

    SN - 1096-6080

    IS - 2

    ER -

    Scown TM, Santos EM, Johnston BD, Gaiser B, Baalousha M, Mitov S et al. Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout. Toxicological Sciences . 2010 Jun;115(2):521-534. https://doi.org/10.1093/toxsci/kfq076