TY - JOUR
T1 - Effect of rapeseed peptide on DNA damage and apoptosis in Hela cells
AU - Xue, Zhaohui
AU - Liu, Zhiwei
AU - Wu, Moucheng
AU - Zhuang, Shiwen
AU - Yu, Wancong
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant no. 30800767).
PY - 2010/9
Y1 - 2010/9
N2 - Rapeseed peptide (RSP), obtained by hydrolyzing rapeseed protein, has anticancer activity. In this study, the effects of RSP on proliferation rate, morphological changes, DNA damage, cell cycle distribution and apoptosis in human cervical carcinoma (Hela) cells were investigated. RSP treatment at a concentration of 640. mg/L for 4 days inhibited Hela cell proliferation significantly, as determined by the MTT assay. We observed a dose-dependent increase in cytotoxicity induced by RSP at 20-640. mg/L. After 4 days of 320. mg/L RSP treatment, typical apoptotic changes were observed by transmission electron microscopy (TEM). Using the comet assay, we found dramatic comet tails, indicating DNA damage by RSP (20-640. mg/L). Moreover, RSP treatment caused inhibition of Hela cell growth, with cycle arrest in the S phase and apoptosis induction. Taken together, the results suggested that rapeseed peptide could be a potential antitumor compound with an apoptotic mode of action.
AB - Rapeseed peptide (RSP), obtained by hydrolyzing rapeseed protein, has anticancer activity. In this study, the effects of RSP on proliferation rate, morphological changes, DNA damage, cell cycle distribution and apoptosis in human cervical carcinoma (Hela) cells were investigated. RSP treatment at a concentration of 640. mg/L for 4 days inhibited Hela cell proliferation significantly, as determined by the MTT assay. We observed a dose-dependent increase in cytotoxicity induced by RSP at 20-640. mg/L. After 4 days of 320. mg/L RSP treatment, typical apoptotic changes were observed by transmission electron microscopy (TEM). Using the comet assay, we found dramatic comet tails, indicating DNA damage by RSP (20-640. mg/L). Moreover, RSP treatment caused inhibition of Hela cell growth, with cycle arrest in the S phase and apoptosis induction. Taken together, the results suggested that rapeseed peptide could be a potential antitumor compound with an apoptotic mode of action.
KW - Apoptosis
KW - Cell cycle
KW - Comet assay
KW - Cytotoxicity
KW - Hela cells
KW - Rapeseed peptide (RSP)
UR - http://www.scopus.com/inward/record.url?scp=77955519188&partnerID=8YFLogxK
U2 - 10.1016/j.etp.2009.06.013
DO - 10.1016/j.etp.2009.06.013
M3 - Article
C2 - 19640692
AN - SCOPUS:77955519188
SN - 0940-2993
VL - 62
SP - 519
EP - 523
JO - Experimental and Toxicologic Pathology
JF - Experimental and Toxicologic Pathology
IS - 5
ER -