Effect of rapeseed peptide on DNA damage and apoptosis in Hela cells

Zhaohui Xue, Zhiwei Liu, Moucheng Wu, Shiwen Zhuang, Wancong Yu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)


Rapeseed peptide (RSP), obtained by hydrolyzing rapeseed protein, has anticancer activity. In this study, the effects of RSP on proliferation rate, morphological changes, DNA damage, cell cycle distribution and apoptosis in human cervical carcinoma (Hela) cells were investigated. RSP treatment at a concentration of 640. mg/L for 4 days inhibited Hela cell proliferation significantly, as determined by the MTT assay. We observed a dose-dependent increase in cytotoxicity induced by RSP at 20-640. mg/L. After 4 days of 320. mg/L RSP treatment, typical apoptotic changes were observed by transmission electron microscopy (TEM). Using the comet assay, we found dramatic comet tails, indicating DNA damage by RSP (20-640. mg/L). Moreover, RSP treatment caused inhibition of Hela cell growth, with cycle arrest in the S phase and apoptosis induction. Taken together, the results suggested that rapeseed peptide could be a potential antitumor compound with an apoptotic mode of action.

Original languageEnglish
Pages (from-to)519-523
Number of pages5
JournalExperimental and Toxicologic Pathology
Issue number5
Publication statusPublished - Sept 2010


  • Apoptosis
  • Cell cycle
  • Comet assay
  • Cytotoxicity
  • Hela cells
  • Rapeseed peptide (RSP)

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Cell Biology


Dive into the research topics of 'Effect of rapeseed peptide on DNA damage and apoptosis in Hela cells'. Together they form a unique fingerprint.

Cite this