Effect of glycine on synaptic transmission at the third order giant synapse of the squids Alloteuthis subulata and Loligo vulgaris

I M Vinogradova, J Zajicek, S Gentile, Euan R Brown

Research output: Contribution to journalArticle

Abstract

Intracellular microelectrode recordings were made from presynaptic and postsynaptic regions of the third order giant synapses of the squids Alloteuthis subulata and Loligo vulgaris. Synaptically generated postsynaptic action potential trains, and excitatory postsynaptic potentials (EPSPs) were reversibly decreased by glycine, beta-alanine or taurine while presynaptic action potentials (APs) were unaltered. Glycine was effective in the presence of strychnine (30-50 muM), NMDA (500 muM), AP-5 (50 muM), CPP (100 muM), or MK 801 (which also had no effect on normal synaptic transmission). The glycine effect was reduced reversibly by D-tubocurarine (100 muM) and blocked by reducing extracellular chloride by 50% with propionate. Excitatory postsynaptic currents (EPSCs) were decreased by glycine addition without altering resting membrane conductance. We postulate that glycine or a glycine like substance provides an excitatory postsynaptic input during synaptic stimulation. Bath addition of glycine desensitises these receptors and decreases the amplitude of the EPSPs and EPSCs. Modulation of this synaptic input may provide an effective mechanism to suppress or potentiate synaptic transmission in the squid giant synapse. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)42-46
Number of pages5
JournalNeuroscience letters
Volume325
Issue number1
DOIs
Publication statusPublished - 31 May 2002

Cite this

@article{bdc1af829db54fa1b81490bdf7a938ba,
title = "Effect of glycine on synaptic transmission at the third order giant synapse of the squids Alloteuthis subulata and Loligo vulgaris",
abstract = "Intracellular microelectrode recordings were made from presynaptic and postsynaptic regions of the third order giant synapses of the squids Alloteuthis subulata and Loligo vulgaris. Synaptically generated postsynaptic action potential trains, and excitatory postsynaptic potentials (EPSPs) were reversibly decreased by glycine, beta-alanine or taurine while presynaptic action potentials (APs) were unaltered. Glycine was effective in the presence of strychnine (30-50 muM), NMDA (500 muM), AP-5 (50 muM), CPP (100 muM), or MK 801 (which also had no effect on normal synaptic transmission). The glycine effect was reduced reversibly by D-tubocurarine (100 muM) and blocked by reducing extracellular chloride by 50{\%} with propionate. Excitatory postsynaptic currents (EPSCs) were decreased by glycine addition without altering resting membrane conductance. We postulate that glycine or a glycine like substance provides an excitatory postsynaptic input during synaptic stimulation. Bath addition of glycine desensitises these receptors and decreases the amplitude of the EPSPs and EPSCs. Modulation of this synaptic input may provide an effective mechanism to suppress or potentiate synaptic transmission in the squid giant synapse. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.",
author = "Vinogradova, {I M} and J Zajicek and S Gentile and Brown, {Euan R}",
year = "2002",
month = "5",
day = "31",
doi = "10.1016/S0304-3940(02)00222-7",
language = "English",
volume = "325",
pages = "42--46",
journal = "Neuroscience letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

Effect of glycine on synaptic transmission at the third order giant synapse of the squids Alloteuthis subulata and Loligo vulgaris. / Vinogradova, I M ; Zajicek, J ; Gentile, S ; Brown, Euan R.

In: Neuroscience letters, Vol. 325, No. 1, 31.05.2002, p. 42-46.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of glycine on synaptic transmission at the third order giant synapse of the squids Alloteuthis subulata and Loligo vulgaris

AU - Vinogradova, I M

AU - Zajicek, J

AU - Gentile, S

AU - Brown, Euan R

PY - 2002/5/31

Y1 - 2002/5/31

N2 - Intracellular microelectrode recordings were made from presynaptic and postsynaptic regions of the third order giant synapses of the squids Alloteuthis subulata and Loligo vulgaris. Synaptically generated postsynaptic action potential trains, and excitatory postsynaptic potentials (EPSPs) were reversibly decreased by glycine, beta-alanine or taurine while presynaptic action potentials (APs) were unaltered. Glycine was effective in the presence of strychnine (30-50 muM), NMDA (500 muM), AP-5 (50 muM), CPP (100 muM), or MK 801 (which also had no effect on normal synaptic transmission). The glycine effect was reduced reversibly by D-tubocurarine (100 muM) and blocked by reducing extracellular chloride by 50% with propionate. Excitatory postsynaptic currents (EPSCs) were decreased by glycine addition without altering resting membrane conductance. We postulate that glycine or a glycine like substance provides an excitatory postsynaptic input during synaptic stimulation. Bath addition of glycine desensitises these receptors and decreases the amplitude of the EPSPs and EPSCs. Modulation of this synaptic input may provide an effective mechanism to suppress or potentiate synaptic transmission in the squid giant synapse. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

AB - Intracellular microelectrode recordings were made from presynaptic and postsynaptic regions of the third order giant synapses of the squids Alloteuthis subulata and Loligo vulgaris. Synaptically generated postsynaptic action potential trains, and excitatory postsynaptic potentials (EPSPs) were reversibly decreased by glycine, beta-alanine or taurine while presynaptic action potentials (APs) were unaltered. Glycine was effective in the presence of strychnine (30-50 muM), NMDA (500 muM), AP-5 (50 muM), CPP (100 muM), or MK 801 (which also had no effect on normal synaptic transmission). The glycine effect was reduced reversibly by D-tubocurarine (100 muM) and blocked by reducing extracellular chloride by 50% with propionate. Excitatory postsynaptic currents (EPSCs) were decreased by glycine addition without altering resting membrane conductance. We postulate that glycine or a glycine like substance provides an excitatory postsynaptic input during synaptic stimulation. Bath addition of glycine desensitises these receptors and decreases the amplitude of the EPSPs and EPSCs. Modulation of this synaptic input may provide an effective mechanism to suppress or potentiate synaptic transmission in the squid giant synapse. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

U2 - 10.1016/S0304-3940(02)00222-7

DO - 10.1016/S0304-3940(02)00222-7

M3 - Article

VL - 325

SP - 42

EP - 46

JO - Neuroscience letters

JF - Neuroscience letters

SN - 0304-3940

IS - 1

ER -