Effect of Clathrin Light Chains on the Stiffness of Clathrin Lattices and Membrane Budding

Philip N. Dannhauser, Mitja Platen, Heike Böning, Huberta Ungewickell, Iwan A T Schaap, Ernst J. Ungewickell

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Clathrin-dependent transport processes require the polymerization of clathrin triskelia into polygonal scaffolds. Together with adapter proteins, clathrin collects cargo and induces membrane bud formation. It is not known to what extent clathrin light chains affect the structural and functional properties of clathrin lattices and the ability of clathrin to deform membranes. To address these issues, we have developed a novel procedure for analyzing clathrin lattice formation on rigid surfaces. We found that lattices can form on adaptor-coated convex-, planar- and even shallow concave surfaces, but the rate of formation and resistance to thermal dissociation of the lattice are greatly enhanced on convex surfaces. Atomic force microscopy on planar clathrin lattices demonstrates that the stiffness of the clathrin lattice is strictly dependent on light chains. The reduced stiffness of the lattice also compromised the ability of clathrin to generate coated buds on the surface of rigid liposomal membranes.

Original languageEnglish
Pages (from-to)519-533
Number of pages15
JournalTraffic
Volume16
Issue number5
DOIs
Publication statusPublished - May 2015

Keywords

  • Atomic force microscopy
  • Curvature
  • Electron microscopy
  • Epsin
  • Hsc70
  • Planar lattices
  • Uncoating

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

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