Easy determination of ploidy level in arabidopsis-thaliana plants by means of pollen size measurement

Thomas  Altmann; Brigitte  Damm; Wolf B Frommer; Thomas  Martin; Peter Christian Morris; Dieter Schweizer; Lothar  Willmitzer; Renate  Schmidt

doi:10.1007/BF00232939

Easy determination of ploidy level in arabidopsis-thaliana plants by means of pollen size measurement

Thomas Altmann, Brigitte Damm, Wolf B Frommer, Thomas Martin, Peter Christian Morris, Dieter Schweizer, Lothar Willmitzer, Renate Schmidt

Research output: Contribution to journal › Article › peer-review

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Abstract

Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.

Original language	English
Pages (from-to)	652-656
Number of pages	5
Journal	Plant Cell Reports
Volume	13
Issue number	11
DOIs	https://doi.org/10.1007/BF00232939
Publication status	Published - Aug 1994

Keywords

frequency
explants
protoplasts
direct gene-transfer
agrobacterium-tumefaciens
pollen size
efficiency
tumefaciens-mediated transformation
mutagenesis
transgenic arabidopsis
ploidy

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10.1007/BF00232939

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title = "Easy determination of ploidy level in arabidopsis-thaliana plants by means of pollen size measurement",

abstract = "Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13\% (root explant transformation), 33\% (cotyledon explant transformation), or 38\% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.",

keywords = "frequency, explants, protoplasts, direct gene-transfer, agrobacterium-tumefaciens, pollen size, efficiency, tumefaciens-mediated transformation, mutagenesis, transgenic arabidopsis, ploidy",

author = "Thomas Altmann and Brigitte Damm and Frommer, \{Wolf B\} and Thomas Martin and Morris, \{Peter Christian\} and Dieter Schweizer and Lothar Willmitzer and Renate Schmidt",

year = "1994",

month = aug,

doi = "10.1007/BF00232939",

language = "English",

volume = "13",

pages = "652--656",

journal = "Plant Cell Reports",

issn = "0721-7714",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "11",

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TY - JOUR

T1 - Easy determination of ploidy level in arabidopsis-thaliana plants by means of pollen size measurement

AU - Altmann, Thomas

AU - Damm, Brigitte

AU - Frommer, Wolf B

AU - Martin, Thomas

AU - Morris, Peter Christian

AU - Schweizer, Dieter

AU - Willmitzer, Lothar

AU - Schmidt, Renate

PY - 1994/8

Y1 - 1994/8

N2 - Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.

AB - Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.

KW - frequency

KW - explants

KW - protoplasts

KW - direct gene-transfer

KW - agrobacterium-tumefaciens

KW - pollen size

KW - efficiency

KW - tumefaciens-mediated transformation

KW - mutagenesis

KW - transgenic arabidopsis

KW - ploidy

U2 - 10.1007/BF00232939

DO - 10.1007/BF00232939

M3 - Article

SN - 0721-7714

VL - 13

SP - 652

EP - 656

JO - Plant Cell Reports

JF - Plant Cell Reports

IS - 11

ER -

Easy determination of ploidy level in arabidopsis-thaliana plants by means of pollen size measurement

Abstract

Keywords

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