Easy determination of ploidy level in arabidopsis-thaliana plants by means of pollen size measurement

Thomas Altmann, Brigitte Damm, Wolf B Frommer, Thomas Martin, Peter Christian Morris, Dieter Schweizer, Lothar Willmitzer, Renate Schmidt

    Research output: Contribution to journalArticle

    Abstract

    Cytogenetic examination of transgenic Arabidopsis thaliana (L.) Heynh. plants obtained by Agrobacterium-mediated gene transfer to cotyledon- and root-explants or by direct gene transfer into protoplasts revealed a high percentage of tetraploid or aneuploid transformants. Depending on the transformation procedure used, 13% (root explant transformation), 33% (cotyledon explant transformation), or 38% (direct gene transfer) of the transformants showed aberrant ploidy levels. A good correlation between the ploidy level of a plant and the size of its pollen grains was observed. This allows quick and simple testing of the ploidy level of transgenic Arabidopsis plants.

    Original languageEnglish
    Pages (from-to)652-656
    Number of pages5
    JournalPlant Cell Reports
    Volume13
    Issue number11
    DOIs
    Publication statusPublished - Aug 1994

    Keywords

    • frequency
    • explants
    • protoplasts
    • direct gene-transfer
    • agrobacterium-tumefaciens
    • pollen size
    • efficiency
    • tumefaciens-mediated transformation
    • mutagenesis
    • transgenic arabidopsis
    • ploidy

    Cite this

    Altmann, T., Damm, B., Frommer, W. B., Martin, T., Morris, P. C., Schweizer, D., Willmitzer, L., & Schmidt, R. (1994). Easy determination of ploidy level in arabidopsis-thaliana plants by means of pollen size measurement. Plant Cell Reports, 13(11), 652-656. https://doi.org/10.1007/BF00232939