Dynamic acoustic field activated cell separation (DAFACS)

G. D. Skotis, D. R. S. Cumming, J. N. Roberts, M. O. Riehle, A. L. Bernassau

Research output: Contribution to journalArticle

14 Citations (Scopus)
71 Downloads (Pure)

Abstract

Advances in diagnostics, cell and stem cell technologies drive the development of application-specific tools for cell and particle separation. Acoustic micro-particle separation offers a promising avenue for high-throughput, label-free, high recovery, cell and particle separation and isolation in regenerative medicine. Here, we demonstrate a novel approach utilizing a dynamic acoustic field that is capable of separating an arbitrary size range of cells. We first demonstrate the method for the separation of particles with different diameters between 6 and 45 mu m and secondly particles of different densities in a heterogeneous medium. The dynamic acoustic field is then used to separate dorsal root ganglion cells. The shearless, label-free and low damage characteristics make this method of manipulation particularly suited for biological applications. Advantages of using a dynamic acoustic field for the separation of cells include its inherent safety and biocompatibility, the possibility to operate over large distances (centimetres), high purity (ratio of particle population, up to 100%), and high efficiency (ratio of separated particles over total number of particles to separate, up to 100%).

Original languageEnglish
Pages (from-to)802-810
Number of pages9
JournalLab on a Chip
Volume15
Issue number3
DOIs
Publication statusPublished - 7 Feb 2015

Keywords

  • ULTRASONIC STANDING WAVES
  • COMPUTER EXPERIMENTS
  • CLASSICAL FLUIDS
  • MANIPULATION
  • MICROFLUIDICS
  • PARTICLES
  • TWEEZERS

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