Dual-beam laser traps in biology and medicine: When one beam is not enough

Graeme Whyte; Franziska Lautenschläger; Moritz Kreysing; Lars Boyde; Andrew Ekpenyong; Ulysse Delabre; Kevin Chalut; Kristian Franze; Jochen Guck

doi:10.1117/12.862928

Dual-beam laser traps in biology and medicine: When one beam is not enough

Graeme Whyte^*
, Franziska Lautenschläger
, Moritz Kreysing
, Lars Boyde
, Andrew Ekpenyong
, Ulysse Delabre
, Kevin Chalut
, Kristian Franze
, Jochen Guck

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Citations (Scopus)

Abstract

Optical traps are nowadays quite ubiquitous in biophysical and biological studies. The term is often used synonymously with optical tweezers, one particular incarnation of optical traps. However, there is another kind of optical trap consisting of two non-focused, counter-propagating laser beams. This dual-beam trap predates optical tweezers by almost two decades and currently experiences a renaissance. The advantages of dual-beam traps include lower intensities on the trapped object, decoupling from imaging optics, and the possibility to trap cells and cell clusters up to 100 microns in diameter. When used for deforming cells this trap is referred to as an optical stretcher. I will review several applications of such traps in biology and medicine for the detection of cancer cells, sorting stem cells, testing light guiding properties of retinal cells and the controlled rotation of cells for single cell tomography.

Original language	English
Title of host publication	Optical Trapping and Optical Micromanipulation VII
Publisher	SPIE
ISBN (Print)	9780819482587
DOIs	https://doi.org/10.1117/12.862928
Publication status	Published - 27 Aug 2010
Event	SPIE NanoScience + Engineering 2010 - San Diego, CA, United States Duration: 1 Aug 2010 → 5 Aug 2010

Publication series

Name	Proceedings of SPIE
Volume	7762
ISSN (Print)	0277-786X

Conference

Conference	SPIE NanoScience + Engineering 2010
Country/Territory	United States
City	San Diego, CA
Period	1/08/10 → 5/08/10

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cell mechanics
Optical cell rotator
Optical stretcher
Optical trap
Optical tweezers
Retina optics
Tomography

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

Access to Document

10.1117/12.862928

Cite this

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title = "Dual-beam laser traps in biology and medicine: When one beam is not enough",

abstract = "Optical traps are nowadays quite ubiquitous in biophysical and biological studies. The term is often used synonymously with optical tweezers, one particular incarnation of optical traps. However, there is another kind of optical trap consisting of two non-focused, counter-propagating laser beams. This dual-beam trap predates optical tweezers by almost two decades and currently experiences a renaissance. The advantages of dual-beam traps include lower intensities on the trapped object, decoupling from imaging optics, and the possibility to trap cells and cell clusters up to 100 microns in diameter. When used for deforming cells this trap is referred to as an optical stretcher. I will review several applications of such traps in biology and medicine for the detection of cancer cells, sorting stem cells, testing light guiding properties of retinal cells and the controlled rotation of cells for single cell tomography.",

keywords = "Cell mechanics, Optical cell rotator, Optical stretcher, Optical trap, Optical tweezers, Retina optics, Tomography",

author = "Graeme Whyte and Franziska Lautenschl{\"a}ger and Moritz Kreysing and Lars Boyde and Andrew Ekpenyong and Ulysse Delabre and Kevin Chalut and Kristian Franze and Jochen Guck",

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day = "27",

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language = "English",

isbn = "9780819482587",

series = "Proceedings of SPIE",

publisher = "SPIE",

booktitle = "Optical Trapping and Optical Micromanipulation VII",

address = "United States",

note = "SPIE NanoScience + Engineering 2010 ; Conference date: 01-08-2010 Through 05-08-2010",

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Whyte, G, Lautenschläger, F, Kreysing, M, Boyde, L, Ekpenyong, A, Delabre, U, Chalut, K, Franze, K & Guck, J 2010, Dual-beam laser traps in biology and medicine: When one beam is not enough. in Optical Trapping and Optical Micromanipulation VII., 77620G, Proceedings of SPIE, vol. 7762, SPIE, SPIE NanoScience + Engineering 2010, San Diego, CA, United States, 1/08/10. https://doi.org/10.1117/12.862928

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T2 - SPIE NanoScience + Engineering 2010

AU - Whyte, Graeme

AU - Lautenschläger, Franziska

AU - Kreysing, Moritz

AU - Boyde, Lars

AU - Ekpenyong, Andrew

AU - Delabre, Ulysse

AU - Chalut, Kevin

AU - Franze, Kristian

AU - Guck, Jochen

PY - 2010/8/27

Y1 - 2010/8/27

N2 - Optical traps are nowadays quite ubiquitous in biophysical and biological studies. The term is often used synonymously with optical tweezers, one particular incarnation of optical traps. However, there is another kind of optical trap consisting of two non-focused, counter-propagating laser beams. This dual-beam trap predates optical tweezers by almost two decades and currently experiences a renaissance. The advantages of dual-beam traps include lower intensities on the trapped object, decoupling from imaging optics, and the possibility to trap cells and cell clusters up to 100 microns in diameter. When used for deforming cells this trap is referred to as an optical stretcher. I will review several applications of such traps in biology and medicine for the detection of cancer cells, sorting stem cells, testing light guiding properties of retinal cells and the controlled rotation of cells for single cell tomography.

AB - Optical traps are nowadays quite ubiquitous in biophysical and biological studies. The term is often used synonymously with optical tweezers, one particular incarnation of optical traps. However, there is another kind of optical trap consisting of two non-focused, counter-propagating laser beams. This dual-beam trap predates optical tweezers by almost two decades and currently experiences a renaissance. The advantages of dual-beam traps include lower intensities on the trapped object, decoupling from imaging optics, and the possibility to trap cells and cell clusters up to 100 microns in diameter. When used for deforming cells this trap is referred to as an optical stretcher. I will review several applications of such traps in biology and medicine for the detection of cancer cells, sorting stem cells, testing light guiding properties of retinal cells and the controlled rotation of cells for single cell tomography.

KW - Cell mechanics

KW - Optical cell rotator

KW - Optical stretcher

KW - Optical trap

KW - Optical tweezers

KW - Retina optics

KW - Tomography

UR - https://www.scopus.com/pages/publications/77958130524

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DO - 10.1117/12.862928

M3 - Conference contribution

AN - SCOPUS:77958130524

SN - 9780819482587

T3 - Proceedings of SPIE

BT - Optical Trapping and Optical Micromanipulation VII

PB - SPIE

Y2 - 1 August 2010 through 5 August 2010

ER -

Dual-beam laser traps in biology and medicine: When one beam is not enough

Abstract

Publication series

Conference

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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