Dry-coated microprojection array patches for targeted delivery of immunotherapeutics to the skin

Xianfeng Chen, Tarl W. Prow, Michael L. Crichton, Derek W K Jenkins, Michael S. Roberts, Ian H. Frazer, Germain J P Fernando, Mark A F Kendall

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Dry-coated microprojections (MPs) deliver vaccine to abundant immunogenic cells within the skin to induce immune responses. Success in this targeted vaccine delivery relies on overcoming the challenges of dry-coating the vaccine onto the very small (≤ 90 μm length) and densely packed (~ 20,000 cm- 2) MPs. In this paper, we show that a gas-jet drying coating method achieves the desired uniform coating. The coating approach is robustly demonstrated on compounds representative of a range of immunotherapeutics (e.g. DNA, proteins), with each uniformly coated on thousands of MPs. Furthermore, the dry-coating remains intact during skin insertion, and then releases within the wet skin cellular environment within 3 min. Finally, we applied ovalbumin protein coated MP patches to immunise mice, achieving comparable antibody levels (p = 0.08) with needle and syringe intramuscular injection. In summary, this paper presents a simple, practical and versatile method to achieve uniform coating on very small and densely packed MPs for a needle-free and targeted vaccine delivery technology.

Original languageEnglish
Pages (from-to)212-220
Number of pages9
JournalJournal of Controlled Release
Volume139
Issue number3
DOIs
Publication statusPublished - 3 Nov 2009

Keywords

  • Gas-jet drying coating
  • Microprojections
  • Targeted vaccine delivery

ASJC Scopus subject areas

  • Pharmaceutical Science

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    Chen, X., Prow, T. W., Crichton, M. L., Jenkins, D. W. K., Roberts, M. S., Frazer, I. H., Fernando, G. J. P., & Kendall, M. A. F. (2009). Dry-coated microprojection array patches for targeted delivery of immunotherapeutics to the skin. Journal of Controlled Release, 139(3), 212-220. https://doi.org/10.1016/j.jconrel.2009.06.029