Drebrin-like protein DBN-1 is a sarcomere component that stabilizes actin filaments during muscle contraction

Eugenia Butkevich, Kai Bodensiek, Nikta Fakhri, Kerstin Von Roden, Iwan A T Schaap, Christoph F. Schmidt, Dieter R. Klopfenstein, Irina Majoul

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Actin filament organization and stability in the sarcomeres of muscle cells are critical for force generation. Here we identify and functionally characterize a Caenorhabditis elegans drebrin-like protein DBN-1 as a novel constituent of the muscle contraction machinery. In vitro, DBN-1 exhibits actin filament binding and bundling activity. In vivo, DBN-1 is expressed in body wall muscles of C. elegans. During the muscle contraction cycle, DBN-1 alternates location between myosin-and actin-rich regions of the sarcomere. In contracted muscle, DBN-1 is accumulated at I-bands where it likely regulates proper spacing of α-actinin and tropomyosin and protects actin filaments from the interaction with ADF/cofilin. DBN-1 loss of function results in the partial depolymerization of F-actin during muscle contraction. Taken together, our data show that DBN-1 organizes the muscle contractile apparatus maintaining the spatial relationship between actin-binding proteins such as α-actinin, tropomyosin and ADF/cofilin and possibly strengthening actin filaments by bundling.

Original languageEnglish
Article number7523
JournalNature Communications
Volume6
DOIs
Publication statusPublished - 6 Jul 2015

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Fingerprint Dive into the research topics of 'Drebrin-like protein DBN-1 is a sarcomere component that stabilizes actin filaments during muscle contraction'. Together they form a unique fingerprint.

Cite this