TY - JOUR
T1 - DNA-SIP reveals an overlooked methanotroph, Crenothrix sp., involved in methane consumption in shallow lake sediments
AU - Yang, Yuyin
AU - Chen, Jianfei
AU - Pratscher, Jennifer
AU - Xie, Shuguang
N1 - Funding Information:
This work was financially supported by the National Natural Science Foundation of China (Nos. 42007295 , 41571444 ), and a NERC Independent Research Fellowship NE/L010771/2 . Data processing was supported by the High-performance Computing Platform of Peking University . We thank Bruce Steel for assistance with SIP.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/3/25
Y1 - 2022/3/25
N2 - Methanotrophs are the main consumers of methane produced in lake sediments. In shallow lakes suffering from eutrophication, methanogenesis is accelerated by the excess organic carbon input, and thus methanotrophs play a key role in regulating this methane flux as well as carbon cycling. Here, we applied nucleic acid stable isotope probing (SIP) to investigate the active methanotrophic microbial community in sediments of several shallow lakes affected by eutrophication. Our results showed that an active methanotrophic community dominated by gamma-proteobacterial methanotrophs, as well as abundant beta-proteobacterial methanol-utilizers, was involved in methane-derived carbon assimilation. Crenothrix, a filamentous methanotroph, was found to be a key methane consumer in all studied lakes. The ecological role of Crenothrix in lacustrine ecosystems is so far poorly understood, with only limited information on its existence in the water column of stratified lakes. Our results provide a novel ecological insight into this group by revealing a wide distribution of Crenothrix in lake sediments. The active methane assimilation by Crenothrix also suggested that it might represent a so far overlooked but crucial biological sink of methane in shallow lakes.
AB - Methanotrophs are the main consumers of methane produced in lake sediments. In shallow lakes suffering from eutrophication, methanogenesis is accelerated by the excess organic carbon input, and thus methanotrophs play a key role in regulating this methane flux as well as carbon cycling. Here, we applied nucleic acid stable isotope probing (SIP) to investigate the active methanotrophic microbial community in sediments of several shallow lakes affected by eutrophication. Our results showed that an active methanotrophic community dominated by gamma-proteobacterial methanotrophs, as well as abundant beta-proteobacterial methanol-utilizers, was involved in methane-derived carbon assimilation. Crenothrix, a filamentous methanotroph, was found to be a key methane consumer in all studied lakes. The ecological role of Crenothrix in lacustrine ecosystems is so far poorly understood, with only limited information on its existence in the water column of stratified lakes. Our results provide a novel ecological insight into this group by revealing a wide distribution of Crenothrix in lake sediments. The active methane assimilation by Crenothrix also suggested that it might represent a so far overlooked but crucial biological sink of methane in shallow lakes.
KW - Crenothrix
KW - DNA-SIP
KW - Lake sediment
KW - Methane-oxidizing bacteria (MOB)
KW - Methanotrophs
UR - http://www.scopus.com/inward/record.url?scp=85122083333&partnerID=8YFLogxK
U2 - 10.1016/j.scitotenv.2021.152742
DO - 10.1016/j.scitotenv.2021.152742
M3 - Article
C2 - 34974014
SN - 0048-9697
VL - 814
JO - Science of the Total Environment
JF - Science of the Total Environment
M1 - 152742
ER -