Distribution of restriction endonucleases among some entomopathogenic strains of Bacillus sphaericus

V. Zahner, F. G. Priest

    Research output: Contribution to journalArticle

    Abstract

    The restriction enzyme BspTI, an isoschizomer of HaeIII (recognition site GGCC), has been detected in eight strains of serotype 5a5b and two serotype 3 strains of the entomopathogenic bacterium Bacillus sphaericus. Strains from other serotypes contained the enzymes BspTII and BspTIII, which digested pBR322 DNA into similar banding patterns after agarose gel electrophoresis but differed in their susceptibility to methylation of the substrate. Strains from serotypes 9, 25 and 26a26b were lacking in restriction enzyme activity. There was little correlation between phage typing and restriction enzyme activity, suggesting that restriction and modification are not responsible for phage specificity among entomopathogenic B. sphaericus strains.

    Original languageEnglish
    Pages (from-to)483-487
    Number of pages5
    JournalLetters in Applied Microbiology
    Volume24
    Issue number6
    Publication statusPublished - 1997

    Fingerprint

    Bacillus sphaericus
    restriction endonucleases
    serotypes
    bacteriophages
    entomopathogenic bacteria
    enzyme activity
    enzymes
    methylation
    agarose
    gel electrophoresis
    DNA

    Cite this

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    abstract = "The restriction enzyme BspTI, an isoschizomer of HaeIII (recognition site GGCC), has been detected in eight strains of serotype 5a5b and two serotype 3 strains of the entomopathogenic bacterium Bacillus sphaericus. Strains from other serotypes contained the enzymes BspTII and BspTIII, which digested pBR322 DNA into similar banding patterns after agarose gel electrophoresis but differed in their susceptibility to methylation of the substrate. Strains from serotypes 9, 25 and 26a26b were lacking in restriction enzyme activity. There was little correlation between phage typing and restriction enzyme activity, suggesting that restriction and modification are not responsible for phage specificity among entomopathogenic B. sphaericus strains.",
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    Distribution of restriction endonucleases among some entomopathogenic strains of Bacillus sphaericus. / Zahner, V.; Priest, F. G.

    In: Letters in Applied Microbiology, Vol. 24, No. 6, 1997, p. 483-487.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Distribution of restriction endonucleases among some entomopathogenic strains of Bacillus sphaericus

    AU - Zahner, V.

    AU - Priest, F. G.

    PY - 1997

    Y1 - 1997

    N2 - The restriction enzyme BspTI, an isoschizomer of HaeIII (recognition site GGCC), has been detected in eight strains of serotype 5a5b and two serotype 3 strains of the entomopathogenic bacterium Bacillus sphaericus. Strains from other serotypes contained the enzymes BspTII and BspTIII, which digested pBR322 DNA into similar banding patterns after agarose gel electrophoresis but differed in their susceptibility to methylation of the substrate. Strains from serotypes 9, 25 and 26a26b were lacking in restriction enzyme activity. There was little correlation between phage typing and restriction enzyme activity, suggesting that restriction and modification are not responsible for phage specificity among entomopathogenic B. sphaericus strains.

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    JF - Letters in Applied Microbiology

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