Differentiation of mosquito-pathogenic strains of Bacillus sphaericus from non-toxic varieties by ribosomal RNA gene restriction patterns

M. Aquino de Muro, W. J. Mitchell, F. G. Priest

Research output: Contribution to journalArticle

Abstract

DNA from 17 strains of Bacillus sphaericus, including representatives of all the established DNA homology groups, was cleaved with EcoRI or HindIII and fragments were separated by agarose gel electrophoresis. Southern blots of this DNA were hybridized to a radioactively labelled DNA probe prepared from the cloned 16S rrnB ribosomal RNA operon of Escherichia coli. Banding patterns of the chromosomal DNA digests and the autoradiograms were specific to DNA homology groups I (B. sphaericus sensu stricto), IIA (mosquito-pathogenic strains), IIB (B. fusiformis) and V, but groups III and IV were not clearly distinguished. This suggests that the mosquito-pathogenic strains represent a separate subspecies.

Original languageEnglish
Pages (from-to)1159-1166
Number of pages8
JournalJournal of General Microbiology
Volume138
Issue number6
Publication statusPublished - 1992

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Bacillus sphaericus
Culicidae
ribosomal RNA
DNA
genes
DNA probes
operon
Southern blotting
agarose
gel electrophoresis
virulent strains
Escherichia coli

Cite this

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Differentiation of mosquito-pathogenic strains of Bacillus sphaericus from non-toxic varieties by ribosomal RNA gene restriction patterns. / Aquino de Muro, M.; Mitchell, W. J.; Priest, F. G.

In: Journal of General Microbiology, Vol. 138, No. 6, 1992, p. 1159-1166.

Research output: Contribution to journalArticle

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AU - Priest, F. G.

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