Differences in magnetic particle uptake by CNS neuroglial subclasses: implications for neural tissue engineering

Stuart I Jenkins, Mark R Pickard, David N Furness, Humphrey Hak Ping Yiu, Divya M Chari

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Aim: To analyze magnetic particle uptake and intracellular processing by the four main non-neuronal subclasses of the CNS: oligodendrocyte precursor cells; oligodendrocytes; astrocytes; and microglia. Materials & methods: Magnetic particle uptake and processing were studied in rat oligodendrocyte precursor cells and oligodendrocytes using fluorescence and transmission electron microscopy, and the results collated with previous data from rat microglia and astrocyte studies. All cells were derived from primary mixed glial cultures. Results: Significant intercellular differences were observed between glial subtypes: microglia demonstrate the most rapid/extensive particle uptake, followed by astrocytes, with oligodendrocyte precursor cells and oligodendrocytes showing significantly lower uptake. Ultrastructural analyses suggest that magnetic particles are extensively degraded in microglia, but relatively stable in other cells. Conclusion: Intercellular differences in particle uptake and handling exist between the major neuroglial subtypes. This has important implications for the utility of the magnetic particle platform for neurobiological applications including genetic modification, transplant cell labeling and biomolecule delivery to mixed CNS cell populations.

Original languageEnglish
Pages (from-to)951-968
Number of pages18
JournalNanomedicine
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 2013

Keywords

  • endocytosis
  • magnetic particle
  • neuroglia
  • oligodendrocyte precursor cell
  • uptake
  • CENTRAL-NERVOUS-SYSTEM
  • MEDIATED GENE-TRANSFER
  • IN-VIVO MRI
  • CELL-LINES
  • CROSS-CONTAMINATION
  • RESONANCE TRACKING
  • STEM-CELLS
  • NANOPARTICLES
  • MICROGLIA
  • OLIGODENDROCYTE

Cite this