Abstract
Probiotic Kocuria SM1, which was part of the allochthonous gut microbiota of rainbow trout, was administered to rainbow trout (Oncorhynchus mykiss) fingerlings as dietary supplement at ~108 cells g-1 for two weeks. The fish were challenged with Vibrio anguillarum and innate immunity determined over the following 5 weeks. The probiotic-fed fish survived (mortalities = 10-28%; P < 0.05) better than the controls (mortalities = 73-92%) for 5 weeks after stopping administration of Kocuria SM1, although the relative percent survival (RPS) declined steadily from 87 to 36% by the end of the experiment. A significant (P < 0.05) effect on the immune parameters was observed in fish within 3 weeks after stopping administration of Kocuria SM1 in comparison with the controls, i.e. enhancement of the respiratory burst (OD550nm) from 0.051 ± 0.014 to 0.067 ± 0.009, lysozyme 605 ± 185 units ml-1 to 872 ± 114 units ml-1, total protein 19.8 ± 2.0 mg ml-1 to 23.8 ± 0.8 mg ml-1 at 2 weeks, and leukocrit 2.9 ± 1.8% to 5.1 ± 1.1%, peroxidase (OD550nm) 0.23 ± 0.02 to 0.37 ± 0.11 and bacterial killing activities (percentage of surviving bacteria = 82 ± 12% to 57 ± 4%) at 3 weeks. Generally, the levels reduced progressively towards the end of experiment. © 2010 Elsevier Ltd.
| Original language | English |
|---|---|
| Pages (from-to) | 212-216 |
| Number of pages | 5 |
| Journal | Fish and Shellfish Immunology |
| Volume | 29 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 2010 |
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Keywords
- Innate defense mechanisms
- Kocuria
- Probiotics
- Rainbow trout
- Vibrio anguillarum
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Development of protection in rainbow trout (Oncorhynchus mykiss, Walbaum) to Vibrio anguillarum following use of the probiotic Kocuria SM1. / Sharifuzzaman, S. M.; Austin, B.
In: Fish and Shellfish Immunology, Vol. 29, No. 2, 08.2010, p. 212-216.Research output: Contribution to journal › Article
TY - JOUR
T1 - Development of protection in rainbow trout (Oncorhynchus mykiss, Walbaum) to Vibrio anguillarum following use of the probiotic Kocuria SM1
AU - Sharifuzzaman, S. M.
AU - Austin, B.
PY - 2010/8
Y1 - 2010/8
N2 - Probiotic Kocuria SM1, which was part of the allochthonous gut microbiota of rainbow trout, was administered to rainbow trout (Oncorhynchus mykiss) fingerlings as dietary supplement at ~108 cells g-1 for two weeks. The fish were challenged with Vibrio anguillarum and innate immunity determined over the following 5 weeks. The probiotic-fed fish survived (mortalities = 10-28%; P < 0.05) better than the controls (mortalities = 73-92%) for 5 weeks after stopping administration of Kocuria SM1, although the relative percent survival (RPS) declined steadily from 87 to 36% by the end of the experiment. A significant (P < 0.05) effect on the immune parameters was observed in fish within 3 weeks after stopping administration of Kocuria SM1 in comparison with the controls, i.e. enhancement of the respiratory burst (OD550nm) from 0.051 ± 0.014 to 0.067 ± 0.009, lysozyme 605 ± 185 units ml-1 to 872 ± 114 units ml-1, total protein 19.8 ± 2.0 mg ml-1 to 23.8 ± 0.8 mg ml-1 at 2 weeks, and leukocrit 2.9 ± 1.8% to 5.1 ± 1.1%, peroxidase (OD550nm) 0.23 ± 0.02 to 0.37 ± 0.11 and bacterial killing activities (percentage of surviving bacteria = 82 ± 12% to 57 ± 4%) at 3 weeks. Generally, the levels reduced progressively towards the end of experiment. © 2010 Elsevier Ltd.
AB - Probiotic Kocuria SM1, which was part of the allochthonous gut microbiota of rainbow trout, was administered to rainbow trout (Oncorhynchus mykiss) fingerlings as dietary supplement at ~108 cells g-1 for two weeks. The fish were challenged with Vibrio anguillarum and innate immunity determined over the following 5 weeks. The probiotic-fed fish survived (mortalities = 10-28%; P < 0.05) better than the controls (mortalities = 73-92%) for 5 weeks after stopping administration of Kocuria SM1, although the relative percent survival (RPS) declined steadily from 87 to 36% by the end of the experiment. A significant (P < 0.05) effect on the immune parameters was observed in fish within 3 weeks after stopping administration of Kocuria SM1 in comparison with the controls, i.e. enhancement of the respiratory burst (OD550nm) from 0.051 ± 0.014 to 0.067 ± 0.009, lysozyme 605 ± 185 units ml-1 to 872 ± 114 units ml-1, total protein 19.8 ± 2.0 mg ml-1 to 23.8 ± 0.8 mg ml-1 at 2 weeks, and leukocrit 2.9 ± 1.8% to 5.1 ± 1.1%, peroxidase (OD550nm) 0.23 ± 0.02 to 0.37 ± 0.11 and bacterial killing activities (percentage of surviving bacteria = 82 ± 12% to 57 ± 4%) at 3 weeks. Generally, the levels reduced progressively towards the end of experiment. © 2010 Elsevier Ltd.
KW - Innate defense mechanisms
KW - Kocuria
KW - Probiotics
KW - Rainbow trout
KW - Vibrio anguillarum
UR - http://www.scopus.com/inward/record.url?scp=77954956363&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2010.03.008
DO - 10.1016/j.fsi.2010.03.008
M3 - Article
VL - 29
SP - 212
EP - 216
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
SN - 1050-4648
IS - 2
ER -