Development of protection in rainbow trout (Oncorhynchus mykiss, Walbaum) to Vibrio anguillarum following use of the probiotic Kocuria SM1

S. M. Sharifuzzaman, B. Austin

    Research output: Contribution to journalArticle

    Abstract

    Probiotic Kocuria SM1, which was part of the allochthonous gut microbiota of rainbow trout, was administered to rainbow trout (Oncorhynchus mykiss) fingerlings as dietary supplement at ~108 cells g-1 for two weeks. The fish were challenged with Vibrio anguillarum and innate immunity determined over the following 5 weeks. The probiotic-fed fish survived (mortalities = 10-28%; P < 0.05) better than the controls (mortalities = 73-92%) for 5 weeks after stopping administration of Kocuria SM1, although the relative percent survival (RPS) declined steadily from 87 to 36% by the end of the experiment. A significant (P < 0.05) effect on the immune parameters was observed in fish within 3 weeks after stopping administration of Kocuria SM1 in comparison with the controls, i.e. enhancement of the respiratory burst (OD550nm) from 0.051 ± 0.014 to 0.067 ± 0.009, lysozyme 605 ± 185 units ml-1 to 872 ± 114 units ml-1, total protein 19.8 ± 2.0 mg ml-1 to 23.8 ± 0.8 mg ml-1 at 2 weeks, and leukocrit 2.9 ± 1.8% to 5.1 ± 1.1%, peroxidase (OD550nm) 0.23 ± 0.02 to 0.37 ± 0.11 and bacterial killing activities (percentage of surviving bacteria = 82 ± 12% to 57 ± 4%) at 3 weeks. Generally, the levels reduced progressively towards the end of experiment. © 2010 Elsevier Ltd.

    Original languageEnglish
    Pages (from-to)212-216
    Number of pages5
    JournalFish and Shellfish Immunology
    Volume29
    Issue number2
    DOIs
    Publication statusPublished - Aug 2010

    Fingerprint

    Vibrio
    Oncorhynchus mykiss
    Probiotics
    Fishes
    Respiratory Burst
    Mortality
    Muramidase
    Dietary Supplements
    Innate Immunity
    Peroxidase
    Bacteria
    Proteins

    Keywords

    • Innate defense mechanisms
    • Kocuria
    • Probiotics
    • Rainbow trout
    • Vibrio anguillarum

    Cite this

    @article{74d8f4b7c61342c29418c494a424423b,
    title = "Development of protection in rainbow trout (Oncorhynchus mykiss, Walbaum) to Vibrio anguillarum following use of the probiotic Kocuria SM1",
    abstract = "Probiotic Kocuria SM1, which was part of the allochthonous gut microbiota of rainbow trout, was administered to rainbow trout (Oncorhynchus mykiss) fingerlings as dietary supplement at ~108 cells g-1 for two weeks. The fish were challenged with Vibrio anguillarum and innate immunity determined over the following 5 weeks. The probiotic-fed fish survived (mortalities = 10-28{\%}; P < 0.05) better than the controls (mortalities = 73-92{\%}) for 5 weeks after stopping administration of Kocuria SM1, although the relative percent survival (RPS) declined steadily from 87 to 36{\%} by the end of the experiment. A significant (P < 0.05) effect on the immune parameters was observed in fish within 3 weeks after stopping administration of Kocuria SM1 in comparison with the controls, i.e. enhancement of the respiratory burst (OD550nm) from 0.051 ± 0.014 to 0.067 ± 0.009, lysozyme 605 ± 185 units ml-1 to 872 ± 114 units ml-1, total protein 19.8 ± 2.0 mg ml-1 to 23.8 ± 0.8 mg ml-1 at 2 weeks, and leukocrit 2.9 ± 1.8{\%} to 5.1 ± 1.1{\%}, peroxidase (OD550nm) 0.23 ± 0.02 to 0.37 ± 0.11 and bacterial killing activities (percentage of surviving bacteria = 82 ± 12{\%} to 57 ± 4{\%}) at 3 weeks. Generally, the levels reduced progressively towards the end of experiment. {\circledC} 2010 Elsevier Ltd.",
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    author = "Sharifuzzaman, {S. M.} and B. Austin",
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    Development of protection in rainbow trout (Oncorhynchus mykiss, Walbaum) to Vibrio anguillarum following use of the probiotic Kocuria SM1. / Sharifuzzaman, S. M.; Austin, B.

    In: Fish and Shellfish Immunology, Vol. 29, No. 2, 08.2010, p. 212-216.

    Research output: Contribution to journalArticle

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