Development of a shuttle vector for screening of strong promoter sequences

Martin Tangney; Wilfrid J. Mitchell; Michael Dolberg-Rasmussen

doi:10.1023/A:1008983208118

Development of a shuttle vector for screening of strong promoter sequences

Martin Tangney, Wilfrid J. Mitchell, Michael Dolberg-Rasmussen

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.

Original language	English
Pages (from-to)	141-144
Number of pages	4
Journal	Biotechnology Techniques
Volume	13
Issue number	2
DOIs	https://doi.org/10.1023/A:1008983208118
Publication status	Published - 1999

Keywords

Gene expression
Promoter probe vector
Transcriptional terminator

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10.1023/A:1008983208118

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title = "Development of a shuttle vector for screening of strong promoter sequences",

abstract = "A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.",

keywords = "Gene expression, Promoter probe vector, Transcriptional terminator",

author = "Martin Tangney and Mitchell, \{Wilfrid J.\} and Michael Dolberg-Rasmussen",

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AU - Tangney, Martin

AU - Mitchell, Wilfrid J.

AU - Dolberg-Rasmussen, Michael

PY - 1999

Y1 - 1999

N2 - A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.

AB - A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.

KW - Gene expression

KW - Promoter probe vector

KW - Transcriptional terminator

UR - https://www.scopus.com/pages/publications/0032999636

U2 - 10.1023/A:1008983208118

DO - 10.1023/A:1008983208118

M3 - Article

SN - 0951-208X

VL - 13

SP - 141

EP - 144

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 2

ER -

Development of a shuttle vector for screening of strong promoter sequences

Abstract

Keywords

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