TY - JOUR
T1 - Development of a shuttle vector for screening of strong promoter sequences
AU - Tangney, Martin
AU - Mitchell, Wilfrid J.
AU - Dolberg-Rasmussen, Michael
PY - 1999
Y1 - 1999
N2 - A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.
AB - A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.
KW - Gene expression
KW - Promoter probe vector
KW - Transcriptional terminator
UR - http://www.scopus.com/inward/record.url?scp=0032999636&partnerID=8YFLogxK
U2 - 10.1023/A:1008983208118
DO - 10.1023/A:1008983208118
M3 - Article
SN - 0951-208X
VL - 13
SP - 141
EP - 144
JO - Biotechnology Techniques
JF - Biotechnology Techniques
IS - 2
ER -