Development of a shuttle vector for screening of strong promoter sequences

Martin Tangney, Wilfrid J. Mitchell, Michael Dolberg-Rasmussen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalBiotechnology Techniques
Volume13
Issue number2
DOIs
Publication statusPublished - 1999

Keywords

  • Gene expression
  • Promoter probe vector
  • Transcriptional terminator

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