Development of a shuttle vector for screening of strong promoter sequences

Martin Tangney, Wilfrid J. Mitchell, Michael Dolberg-Rasmussen

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)


    A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.

    Original languageEnglish
    Pages (from-to)141-144
    Number of pages4
    JournalBiotechnology Techniques
    Issue number2
    Publication statusPublished - 1999


    • Gene expression
    • Promoter probe vector
    • Transcriptional terminator


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