Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

Ana Isabel dos Ramos Catarino; Richard Thompson; William Sanderson; Theodore B Henry

doi:10.1002/etc.3608

Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

Ana Isabel dos Ramos Catarino, Richard Thompson, William Sanderson, Theodore B Henry

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Abstract

The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH and
enzyme digestions. The authors recommend use of industrial enzymes based on digestion efﬁciency, microplastic recovery, and avoidance of caustic chemicals.

Original language	English
Pages (from-to)	947–951
Number of pages	5
Journal	Environmental Toxicology and Chemistry
Volume	36
Issue number	4
Early online date	1 Sept 2016
DOIs	https://doi.org/10.1002/etc.3608
Publication status	Published - Apr 2017

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Theodore B. Henry
- T.Henryhw.acuk
- School of Energy, Geoscience, Infrastructure and Society - Professor
- School of Energy, Geoscience, Infrastructure and Society, Institute for Life and Earth Sciences - Professor
Person: Academic (Research & Teaching)

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title = "Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues",

abstract = "The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH andenzyme digestions. The authors recommend use of industrial enzymes based on digestion efﬁciency, microplastic recovery, and avoidance of caustic chemicals.",

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AB - The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH andenzyme digestions. The authors recommend use of industrial enzymes based on digestion efﬁciency, microplastic recovery, and avoidance of caustic chemicals.

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Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

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