Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

Ana Isabel dos Ramos Catarino; Richard Thompson; William Sanderson; Theodore B Henry

doi:10.1002/etc.3608

Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

Ana Isabel dos Ramos Catarino, Richard Thompson, William Sanderson, Theodore B Henry

Research output: Contribution to journal › Article › peer-review

82 Citations (Scopus)

215 Downloads (Pure)

Abstract

The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH and
enzyme digestions. The authors recommend use of industrial enzymes based on digestion efﬁciency, microplastic recovery, and avoidance of caustic chemicals.

Original language	English
Pages (from-to)	947–951
Number of pages	5
Journal	Environmental Toxicology and Chemistry
Volume	36
Issue number	4
Early online date	1 Sep 2016
DOIs	https://doi.org/10.1002/etc.3608
Publication status	Published - Apr 2017

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title = "Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues",

abstract = "The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH andenzyme digestions. The authors recommend use of industrial enzymes based on digestion efﬁciency, microplastic recovery, and avoidance of caustic chemicals.",

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Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues. / dos Ramos Catarino, Ana Isabel; Thompson, Richard; Sanderson, William ; Henry, Theodore B.

In: Environmental Toxicology and Chemistry, Vol. 36, No. 4, 04.2017, p. 947–951.

Research output: Contribution to journal › Article › peer-review

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AU - Sanderson, William

AU - Henry, Theodore B

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AB - The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH andenzyme digestions. The authors recommend use of industrial enzymes based on digestion efﬁciency, microplastic recovery, and avoidance of caustic chemicals.

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