Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

Ana Isabel dos Ramos Catarino; Richard Thompson; William Sanderson; Theodore B Henry

doi:10.1002/etc.3608

Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

Ana Isabel dos Ramos Catarino, Richard Thompson, William Sanderson, Theodore B Henry

Research output: Contribution to journal › Article › peer-review

92 Citations (Scopus)

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Abstract

The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH and
enzyme digestions. The authors recommend use of industrial enzymes based on digestion efﬁciency, microplastic recovery, and avoidance of caustic chemicals.

Original language	English
Pages (from-to)	947–951
Number of pages	5
Journal	Environmental Toxicology and Chemistry
Volume	36
Issue number	4
Early online date	1 Sep 2016
DOIs	https://doi.org/10.1002/etc.3608
Publication status	Published - Apr 2017

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Theodore B. Henry
- T.Henry@hw.ac.uk
- School of Energy, Geoscience, Infrastructure and Society - Professor
- School of Energy, Geoscience, Infrastructure and Society, Institute for Life and Earth Sciences - Professor
Person: Academic (Research & Teaching)

Cite this

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title = "Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues",

abstract = "The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH andenzyme digestions. The authors recommend use of industrial enzymes based on digestion efﬁciency, microplastic recovery, and avoidance of caustic chemicals.",

author = "{dos Ramos Catarino}, {Ana Isabel} and Richard Thompson and William Sanderson and Henry, {Theodore B}",

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pages = "947–951",

journal = "Environmental Toxicology and Chemistry",

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Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues. / dos Ramos Catarino, Ana Isabel; Thompson, Richard; Sanderson, William ; Henry, Theodore B.

In: Environmental Toxicology and Chemistry, Vol. 36, No. 4, 04.2017, p. 947–951.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

AU - dos Ramos Catarino, Ana Isabel

AU - Thompson, Richard

AU - Sanderson, William

AU - Henry, Theodore B

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Y1 - 2017/4

N2 - The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH andenzyme digestions. The authors recommend use of industrial enzymes based on digestion efﬁciency, microplastic recovery, and avoidance of caustic chemicals.

AB - The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH andenzyme digestions. The authors recommend use of industrial enzymes based on digestion efﬁciency, microplastic recovery, and avoidance of caustic chemicals.

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DO - 10.1002/etc.3608

M3 - Article

C2 - 27583696

VL - 36

SP - 947

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JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

IS - 4

ER -

Development and optimisation of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

Abstract

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Theodore B. Henry

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