Determination of the orientation of the axial ligands and of the magnetic properties of the haems in the tetrahaem ferricytochrome from Shewanella frigidimarina

Ricardo O Louro, Miguel Pessanha, Graeme A Reid, Stephen K Chapman, David L Turner, Carlos A Salgueiro

    Research output: Contribution to journalArticle

    Abstract

    The unambiguous assignment of the nuclear magnetic resonance (NMR) signals of the (alpha-substituents of the haems in the tetrahaem cytochrome isolated from Shewanella frigidimarina NCIMB400, was made using a combination of homonuclear and heteronuclear experiments. The paramagnetic C-13 shifts of the nuclei directly bound to the porphyrin of each haem group were analysed in the framework of a model for the haern electronic structure. The analysis yields g-tensors for each haem, which allowed the assignment of some electron paramagnetic resonance (EPR) signals to specific haems, and the orientation of the magnetic axes relative to each haem to be established. The orientation of the axial ligands of the haems was determined semi-empirically from the NMR data, and the structural results were compared with those of the homologous tetrahaern cytochrome from Shewanella oneidensis MR-1 showing significant similarities between the two proteins. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

    Original languageEnglish
    Pages (from-to)520-524
    Number of pages5
    JournalFEBS Letters
    Volume531
    Issue number3
    DOIs
    Publication statusPublished - 20 Nov 2002

    Keywords

    • electron transfer
    • SPIN FERRIC PORPHYRINS
    • SHIFTS
    • heteronuclear nuclear magnetic resonance
    • haem electronic structure
    • SUSCEPTIBILITY TENSORS
    • Shewanella
    • PUTREFACIENS MR-1
    • HEME ELECTRONIC-STRUCTURE
    • paramagnetic shift
    • PROTON TRANSLOCATION
    • PROTEINS
    • NMR
    • FUMARATE REDUCTASE
    • CYTOCHROME C(3)

    Cite this

    Louro, Ricardo O ; Pessanha, Miguel ; Reid, Graeme A ; Chapman, Stephen K ; Turner, David L ; Salgueiro, Carlos A. / Determination of the orientation of the axial ligands and of the magnetic properties of the haems in the tetrahaem ferricytochrome from Shewanella frigidimarina. In: FEBS Letters. 2002 ; Vol. 531, No. 3. pp. 520-524.
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    abstract = "The unambiguous assignment of the nuclear magnetic resonance (NMR) signals of the (alpha-substituents of the haems in the tetrahaem cytochrome isolated from Shewanella frigidimarina NCIMB400, was made using a combination of homonuclear and heteronuclear experiments. The paramagnetic C-13 shifts of the nuclei directly bound to the porphyrin of each haem group were analysed in the framework of a model for the haern electronic structure. The analysis yields g-tensors for each haem, which allowed the assignment of some electron paramagnetic resonance (EPR) signals to specific haems, and the orientation of the magnetic axes relative to each haem to be established. The orientation of the axial ligands of the haems was determined semi-empirically from the NMR data, and the structural results were compared with those of the homologous tetrahaern cytochrome from Shewanella oneidensis MR-1 showing significant similarities between the two proteins. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.",
    keywords = "electron transfer, SPIN FERRIC PORPHYRINS, SHIFTS, heteronuclear nuclear magnetic resonance, haem electronic structure, SUSCEPTIBILITY TENSORS, Shewanella, PUTREFACIENS MR-1, HEME ELECTRONIC-STRUCTURE, paramagnetic shift, PROTON TRANSLOCATION, PROTEINS, NMR, FUMARATE REDUCTASE, CYTOCHROME C(3)",
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    Determination of the orientation of the axial ligands and of the magnetic properties of the haems in the tetrahaem ferricytochrome from Shewanella frigidimarina. / Louro, Ricardo O; Pessanha, Miguel; Reid, Graeme A; Chapman, Stephen K; Turner, David L; Salgueiro, Carlos A.

    In: FEBS Letters, Vol. 531, No. 3, 20.11.2002, p. 520-524.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Determination of the orientation of the axial ligands and of the magnetic properties of the haems in the tetrahaem ferricytochrome from Shewanella frigidimarina

    AU - Louro, Ricardo O

    AU - Pessanha, Miguel

    AU - Reid, Graeme A

    AU - Chapman, Stephen K

    AU - Turner, David L

    AU - Salgueiro, Carlos A

    PY - 2002/11/20

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    N2 - The unambiguous assignment of the nuclear magnetic resonance (NMR) signals of the (alpha-substituents of the haems in the tetrahaem cytochrome isolated from Shewanella frigidimarina NCIMB400, was made using a combination of homonuclear and heteronuclear experiments. The paramagnetic C-13 shifts of the nuclei directly bound to the porphyrin of each haem group were analysed in the framework of a model for the haern electronic structure. The analysis yields g-tensors for each haem, which allowed the assignment of some electron paramagnetic resonance (EPR) signals to specific haems, and the orientation of the magnetic axes relative to each haem to be established. The orientation of the axial ligands of the haems was determined semi-empirically from the NMR data, and the structural results were compared with those of the homologous tetrahaern cytochrome from Shewanella oneidensis MR-1 showing significant similarities between the two proteins. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

    AB - The unambiguous assignment of the nuclear magnetic resonance (NMR) signals of the (alpha-substituents of the haems in the tetrahaem cytochrome isolated from Shewanella frigidimarina NCIMB400, was made using a combination of homonuclear and heteronuclear experiments. The paramagnetic C-13 shifts of the nuclei directly bound to the porphyrin of each haem group were analysed in the framework of a model for the haern electronic structure. The analysis yields g-tensors for each haem, which allowed the assignment of some electron paramagnetic resonance (EPR) signals to specific haems, and the orientation of the magnetic axes relative to each haem to be established. The orientation of the axial ligands of the haems was determined semi-empirically from the NMR data, and the structural results were compared with those of the homologous tetrahaern cytochrome from Shewanella oneidensis MR-1 showing significant similarities between the two proteins. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

    KW - electron transfer

    KW - SPIN FERRIC PORPHYRINS

    KW - SHIFTS

    KW - heteronuclear nuclear magnetic resonance

    KW - haem electronic structure

    KW - SUSCEPTIBILITY TENSORS

    KW - Shewanella

    KW - PUTREFACIENS MR-1

    KW - HEME ELECTRONIC-STRUCTURE

    KW - paramagnetic shift

    KW - PROTON TRANSLOCATION

    KW - PROTEINS

    KW - NMR

    KW - FUMARATE REDUCTASE

    KW - CYTOCHROME C(3)

    U2 - 10.1016/S0014-5793(02)03610-4

    DO - 10.1016/S0014-5793(02)03610-4

    M3 - Article

    VL - 531

    SP - 520

    EP - 524

    JO - FEBS Letters

    JF - FEBS Letters

    SN - 0014-5793

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    ER -