Determination of poly(ethylene glycol)s by both normal-phase and reversed-phase modes of high-performance liquid chromatography

Cheng Sun, Margot Baird, Jeni Simpson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A normal-phase HPLC system using an amino column has been developed to characterise oligomers of poly(ethylene glycol)s (PEGs) of average M(r) 400 to 2000 with derivatisation by dinitrobenzoate. Normal-phase HPLC with gradient elution using ternary solvents of hexane, dichloromethane and methanol has produced a baseline resolution for oligomers of PEG 400, 600 and 1000, while PEG 1000 and 2000 were analysed by using binary solvents of acetonitrile and water. Mixtures of PEGs have been determined by these HPLC systems. PEG 400 in a textile finish has also been determined with satisfactory recovery. It has been found that the hydroxyl group of solvents in normal-phase HPLC plays an important role in resolution and retention of PEG oligomers. Derivatisation efficiency for PEGs by dinitrobenzoyl chloride and quantitative determination of derivatised PEGs by HPLC have been studied. A reversed-phase (RP) mode of HPLC was examined determination of PEG 400 oligomers. The normal-phase system provided greater resolution for oligomers of PEGs.

Original languageEnglish
Pages (from-to)231-238
Number of pages8
JournalJournal of Chromatography A
Volume800
Issue number2
DOIs
Publication statusPublished - 27 Mar 1998

Keywords

  • Poly(ethylene glycol)
  • Polymers

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