TY - JOUR
T1 - Detection of Cryptosporidium in miniaturised fluidic devices
AU - Bridle, Helen
AU - Kersaudy-Kerhoas, Maïwenn
AU - Miller, Brian
AU - Gavriilidou, Despoina
AU - Katzer, Frank
AU - Innes, Elisabeth A.
AU - Desmulliez, Marc P. Y.
N1 - Copyright © 2012 Elsevier Ltd. All rights reserved.
PY - 2012/4/15
Y1 - 2012/4/15
N2 - Contamination of drinking water with the protozoan pathogen, Cryptosporidium, represents a serious risk to human health due to the low infectious dose and the resistance of this parasite to chlorine disinfection. Therefore, several countries have legislated for the frequent monitoring of drinking water for Cryptosporidium presence. Existing approved monitoring protocols are however time-consuming and do not provide essential information on the species, virulence or viability of detected oocysts. Rapid, more information-rich and automatable systems for Cryptosporidium detection are highly sought-after, and numerous miniaturised devices have been developed to address this need. This review article aims to summarise the state-of-the-art and compare the performance of these systems in terms of detection limit, ability to determine species, viability and performance in the presence of interferents. Finally, conclusions are drawn with regard to the most promising methods and directions of future research. (C) 2012 Elsevier Ltd. All rights reserved.
AB - Contamination of drinking water with the protozoan pathogen, Cryptosporidium, represents a serious risk to human health due to the low infectious dose and the resistance of this parasite to chlorine disinfection. Therefore, several countries have legislated for the frequent monitoring of drinking water for Cryptosporidium presence. Existing approved monitoring protocols are however time-consuming and do not provide essential information on the species, virulence or viability of detected oocysts. Rapid, more information-rich and automatable systems for Cryptosporidium detection are highly sought-after, and numerous miniaturised devices have been developed to address this need. This review article aims to summarise the state-of-the-art and compare the performance of these systems in terms of detection limit, ability to determine species, viability and performance in the presence of interferents. Finally, conclusions are drawn with regard to the most promising methods and directions of future research. (C) 2012 Elsevier Ltd. All rights reserved.
KW - Parasite
KW - Cryptosporidium
KW - Detection
KW - Miniaturisation
KW - Microfluidics
U2 - 10.1016/j.watres.2012.01.010
DO - 10.1016/j.watres.2012.01.010
M3 - Literature review
C2 - 22305660
SN - 0043-1354
VL - 46
SP - 1641
EP - 1661
JO - Water Research
JF - Water Research
IS - 6
ER -
