Dependence of rat spot14 promoter activity on NF-Y binding to the inverted CCAAT-element at - 100

Karim Roder; Siegmund S. Wolf; Michael Schweizer

doi:10.1271/bbb.64.848

Dependence of rat spot14 promoter activity on NF-Y binding to the inverted CCAAT-element at - 100

Karim Roder
, Siegmund S. Wolf
, Michael Schweizer

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Electrophoretic mobility shift assay (EMSA) and in vitro transcription/translation show that NF-Y binds to the inverted CCAAT-element in the promoter of the rodent spot14 gene. The NF-Y-binding sequence has been shown to be responsible for basal activity in H4IIE. Given the similar role found for the inverted CCAAT-element in the promoter of the FAS gene, NF-Y may have an important function in the control of lipogenesis.

Original language	English
Pages (from-to)	848-851
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	64
Issue number	4
DOIs	https://doi.org/10.1271/bbb.64.848
Publication status	Published - Apr 2000

Keywords

Fatty acid synthase
Inverted CCAAT-element
NF-Y
Spot14 promoter

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abstract = "Electrophoretic mobility shift assay (EMSA) and in vitro transcription/translation show that NF-Y binds to the inverted CCAAT-element in the promoter of the rodent spot14 gene. The NF-Y-binding sequence has been shown to be responsible for basal activity in H4IIE. Given the similar role found for the inverted CCAAT-element in the promoter of the FAS gene, NF-Y may have an important function in the control of lipogenesis.",

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AB - Electrophoretic mobility shift assay (EMSA) and in vitro transcription/translation show that NF-Y binds to the inverted CCAAT-element in the promoter of the rodent spot14 gene. The NF-Y-binding sequence has been shown to be responsible for basal activity in H4IIE. Given the similar role found for the inverted CCAAT-element in the promoter of the FAS gene, NF-Y may have an important function in the control of lipogenesis.

KW - Fatty acid synthase

KW - Inverted CCAAT-element

KW - NF-Y

KW - Spot14 promoter

U2 - 10.1271/bbb.64.848

DO - 10.1271/bbb.64.848

M3 - Article

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VL - 64

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JF - Bioscience, Biotechnology and Biochemistry

IS - 4

ER -

Dependence of rat spot14 promoter activity on NF-Y binding to the inverted CCAAT-element at - 100

Abstract

Keywords

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