Dependence of rat spot14 promoter activity on NF-Y binding to the inverted CCAAT-element at - 100

Karim Roder, Siegmund S. Wolf, Michael Schweizer

    Research output: Contribution to journalArticle

    Abstract

    Electrophoretic mobility shift assay (EMSA) and in vitro transcription/translation show that NF-Y binds to the inverted CCAAT-element in the promoter of the rodent spot14 gene. The NF-Y-binding sequence has been shown to be responsible for basal activity in H4IIE. Given the similar role found for the inverted CCAAT-element in the promoter of the FAS gene, NF-Y may have an important function in the control of lipogenesis.

    Original languageEnglish
    Pages (from-to)848-851
    Number of pages4
    JournalBioscience, Biotechnology and Biochemistry
    Volume64
    Issue number4
    DOIs
    Publication statusPublished - Apr 2000

    Fingerprint

    Lipogenesis
    Electrophoretic Mobility Shift Assay
    Genes
    Rodentia
    In Vitro Techniques

    Keywords

    • Fatty acid synthase
    • Inverted CCAAT-element
    • NF-Y
    • Spot14 promoter

    Cite this

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    title = "Dependence of rat spot14 promoter activity on NF-Y binding to the inverted CCAAT-element at - 100",
    abstract = "Electrophoretic mobility shift assay (EMSA) and in vitro transcription/translation show that NF-Y binds to the inverted CCAAT-element in the promoter of the rodent spot14 gene. The NF-Y-binding sequence has been shown to be responsible for basal activity in H4IIE. Given the similar role found for the inverted CCAAT-element in the promoter of the FAS gene, NF-Y may have an important function in the control of lipogenesis.",
    keywords = "Fatty acid synthase, Inverted CCAAT-element, NF-Y, Spot14 promoter",
    author = "Karim Roder and Wolf, {Siegmund S.} and Michael Schweizer",
    year = "2000",
    month = "4",
    doi = "10.1271/bbb.64.848",
    language = "English",
    volume = "64",
    pages = "848--851",
    journal = "Bioscience, Biotechnology and Biochemistry",
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    publisher = "Japan Society for Bioscience Biotechnology and Agrochemistry",
    number = "4",

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    Dependence of rat spot14 promoter activity on NF-Y binding to the inverted CCAAT-element at - 100. / Roder, Karim; Wolf, Siegmund S.; Schweizer, Michael.

    In: Bioscience, Biotechnology and Biochemistry, Vol. 64, No. 4, 04.2000, p. 848-851.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Dependence of rat spot14 promoter activity on NF-Y binding to the inverted CCAAT-element at - 100

    AU - Roder, Karim

    AU - Wolf, Siegmund S.

    AU - Schweizer, Michael

    PY - 2000/4

    Y1 - 2000/4

    N2 - Electrophoretic mobility shift assay (EMSA) and in vitro transcription/translation show that NF-Y binds to the inverted CCAAT-element in the promoter of the rodent spot14 gene. The NF-Y-binding sequence has been shown to be responsible for basal activity in H4IIE. Given the similar role found for the inverted CCAAT-element in the promoter of the FAS gene, NF-Y may have an important function in the control of lipogenesis.

    AB - Electrophoretic mobility shift assay (EMSA) and in vitro transcription/translation show that NF-Y binds to the inverted CCAAT-element in the promoter of the rodent spot14 gene. The NF-Y-binding sequence has been shown to be responsible for basal activity in H4IIE. Given the similar role found for the inverted CCAAT-element in the promoter of the FAS gene, NF-Y may have an important function in the control of lipogenesis.

    KW - Fatty acid synthase

    KW - Inverted CCAAT-element

    KW - NF-Y

    KW - Spot14 promoter

    U2 - 10.1271/bbb.64.848

    DO - 10.1271/bbb.64.848

    M3 - Article

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    JO - Bioscience, Biotechnology and Biochemistry

    JF - Bioscience, Biotechnology and Biochemistry

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