Decomposition of tobacco roots with modified phenylpropanoid content by fungi with contrasting lignocellulose degradation strategies

E L Tilston, C Halpin, David William Hopkins

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    The decomposition of tobacco roots with genetic modifications to lignin biosynthesis by the ligninolytic fungus Phanerochaete chrysosporium, by the cellulolytic fungus Chaetomium globosum, and by microbial communities in soil were examined to determine whether the rates of decomposition of the modified and unmodified roots decomposed at different rates, whether the order of colonization by P. chrysosporium and C. globosum facilitated decomposition, and whether the microbial community in soil was conditioned by exposure to roots subsequently so that the subsequent decomposition of the roots was increased. Both P. chrysosporium and C. globosum decomposed the modified roots more rapidly, at least initially, than the unmodified roots. Colonization by P. chrysosporium facilitated the subsequent decomposition by C. globosum, presumably because by degrading lignin, P. chrysosporium increased the susceptibility of the polysaccharide component of root material to attack by C. globosum. Selection of the soil microbial community by exposure to the modified residues accelerated subsequent decomposition of the root modified. Although demonstrating effects of the lignin modification on decomposition, they are relatively subtle and in most cases short-lived (less than 40 days) ones to which the microbial community is able to adapt, and therefore, we conclude that there are unlikely to be any persistent effects of the modified lignin on the soil decomposer community.

    Original languageEnglish
    Pages (from-to)305-311
    Number of pages7
    JournalBiology and Fertility of Soils
    Volume49
    Issue number3
    DOIs
    Publication statusPublished - 1 Apr 2013

    Keywords

    • Chaetomium globosum
    • Genetic modification
    • Lignin
    • Tobacco
    • Phanerochaete chrysosporium
    • CINNAMYL ALCOHOL-DEHYDROGENASE
    • LIGNIN BIOSYNTHESIS
    • GENETIC MODIFICATIONS
    • DOWN-REGULATION
    • PHANEROCHAETE-CHRYSOSPORIUM
    • SOIL
    • RESIDUES
    • PLANTS
    • TREES
    • BIOMASS

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