D-aspartic acid in the nervous system of Aplysia limacina: possible role in neurotransmission

Patrizia Spinelli, Euan R Brown, Gabriele Ferrandino, Margherita Branno, Pier Giorgio Montarolo, Enrico D'Aniello, Rakesh K Rastogi, Biagio D'Aniello, Gabriella Chieffi Baccari, George Fisher, Antimo D'Aniello

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Abstract

In the marine mollusk Aplysia limacina, a substantial amount of endogenous D-aspartic acid (D-Asp) was found following its synthesis from L-aspartate by an aspartate racemase. Concentrations Of D-Asp between 3.9 and 4.6 mu mol/g tissue were found in the cerebral, abdominal, buccal, pleural, and pedal ganglia. In non nervous tissues, D-Asp occurred at a very low concentration compared to the nervous system. Immunohistochemical studies conducted on cultured Aplysia neurons using an anti-D-aspartate antibody demonstrated that D-Asp occurs in the soma, dendrites, and in synaptic varicosities. Synaptosomes and synaptic vesicles from cerebral ganglia were prepared and characterized by electron microscopy. HPLC analysis revealed high concentrations Of D-Asp together with L-aspartate and L-glutamate in isolated synaptosomes In addition, D-Asp was released from synaptosomes by K+ depolarization or by ionomycin. D-Asp was one of the principal amino acids present in synaptic vesicles representing about the 25% of total amino acids present in these cellular organelles. injection Of D-Asp into live animals or addition to the incubation media of cultured neurons, caused an increase in cAMP content. Taken as a whole, these findings suggest a possible role Of D-Asp in neurotransmission in the nervous system of Aplysia limacina.

Original languageEnglish
Pages (from-to)672-681
Number of pages10
JournalJournal of Cellular Physiology
Volume206
Issue number3
DOIs
Publication statusPublished - Mar 2006

Cite this

Spinelli, P., Brown, E. R., Ferrandino, G., Branno, M., Montarolo, P. G., D'Aniello, E., Rastogi, R. K., D'Aniello, B., Baccari, G. C., Fisher, G., & D'Aniello, A. (2006). D-aspartic acid in the nervous system of Aplysia limacina: possible role in neurotransmission. Journal of Cellular Physiology, 206(3), 672-681. https://doi.org/10.1002/jcp.20513