Cytokine expression in leucocytes and gut cells of rainbow trout, Oncorhynchus mykiss Walbaum, induced by probiotics

Do Hyung Kim; Brian Austin

doi:10.1016/j.vetimm.2006.08.015

Cytokine expression in leucocytes and gut cells of rainbow trout, Oncorhynchus mykiss Walbaum, induced by probiotics

Do Hyung Kim, Brian Austin

Research output: Contribution to journal › Article › peer-review

139 Citations (Scopus)

Abstract

Understanding how the various host cells respond to probiotic bacteria in vitro may provide important insight into elaborate immune responses triggered by beneficial bacteria. The aim of this study was to investigate the detailed pattern of the mRNA expression of cytokines (IL-1ß, IL-8, TNF-a and TGF-ß) in head kidney (HK) leucocytes and gut cells isolated from rainbow trout (Oncorhynchus mykiss Walbaum) after co-culturing with live probiotics. HK leucocytes and gut cells adjusted to 5 × 10⁶ and 2 × 10⁶ ml^-1, respectively, in L-15 medium containing 25% decomplemented FCS and 300 mg l^-1 l-glutamine were co-cultured with Carnobacterium maltaromaticum B26 and C. divergens B33 at an multiplicity of infection of 25 for 6 and 12 h. Quantitative real-time reverse transcriptase polymerase chain reaction using SYBR Green I was employed to determine the mRNA expression of studied genes. Although neither probiotic strains significantly induced mRNA of the cytokines in gut cells, expression ratios of IL-1ß and TNF-a of HK cells were significantly higher, suggesting that these bacteria can stimulate innate immunity in rainbow trout. © 2006 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	297-304
Number of pages	8
Journal	Veterinary Immunology and Immunopathology
Volume	114
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetimm.2006.08.015
Publication status	Published - 15 Dec 2006

Keywords

Cytokines
mRNA
Probiotics
Rainbow trout
Real-time PCR

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title = "Cytokine expression in leucocytes and gut cells of rainbow trout, Oncorhynchus mykiss Walbaum, induced by probiotics",

abstract = "Understanding how the various host cells respond to probiotic bacteria in vitro may provide important insight into elaborate immune responses triggered by beneficial bacteria. The aim of this study was to investigate the detailed pattern of the mRNA expression of cytokines (IL-1{\ss}, IL-8, TNF-a and TGF-{\ss}) in head kidney (HK) leucocytes and gut cells isolated from rainbow trout (Oncorhynchus mykiss Walbaum) after co-culturing with live probiotics. HK leucocytes and gut cells adjusted to 5 × 106 and 2 × 106 ml-1, respectively, in L-15 medium containing 25% decomplemented FCS and 300 mg l-1 l-glutamine were co-cultured with Carnobacterium maltaromaticum B26 and C. divergens B33 at an multiplicity of infection of 25 for 6 and 12 h. Quantitative real-time reverse transcriptase polymerase chain reaction using SYBR Green I was employed to determine the mRNA expression of studied genes. Although neither probiotic strains significantly induced mRNA of the cytokines in gut cells, expression ratios of IL-1{\ss} and TNF-a of HK cells were significantly higher, suggesting that these bacteria can stimulate innate immunity in rainbow trout. {\textcopyright} 2006 Elsevier B.V. All rights reserved.",

keywords = "Cytokines, mRNA, Probiotics, Rainbow trout, Real-time PCR",

author = "Kim, {Do Hyung} and Brian Austin",

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doi = "10.1016/j.vetimm.2006.08.015",

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AU - Austin, Brian

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N2 - Understanding how the various host cells respond to probiotic bacteria in vitro may provide important insight into elaborate immune responses triggered by beneficial bacteria. The aim of this study was to investigate the detailed pattern of the mRNA expression of cytokines (IL-1ß, IL-8, TNF-a and TGF-ß) in head kidney (HK) leucocytes and gut cells isolated from rainbow trout (Oncorhynchus mykiss Walbaum) after co-culturing with live probiotics. HK leucocytes and gut cells adjusted to 5 × 106 and 2 × 106 ml-1, respectively, in L-15 medium containing 25% decomplemented FCS and 300 mg l-1 l-glutamine were co-cultured with Carnobacterium maltaromaticum B26 and C. divergens B33 at an multiplicity of infection of 25 for 6 and 12 h. Quantitative real-time reverse transcriptase polymerase chain reaction using SYBR Green I was employed to determine the mRNA expression of studied genes. Although neither probiotic strains significantly induced mRNA of the cytokines in gut cells, expression ratios of IL-1ß and TNF-a of HK cells were significantly higher, suggesting that these bacteria can stimulate innate immunity in rainbow trout. © 2006 Elsevier B.V. All rights reserved.

AB - Understanding how the various host cells respond to probiotic bacteria in vitro may provide important insight into elaborate immune responses triggered by beneficial bacteria. The aim of this study was to investigate the detailed pattern of the mRNA expression of cytokines (IL-1ß, IL-8, TNF-a and TGF-ß) in head kidney (HK) leucocytes and gut cells isolated from rainbow trout (Oncorhynchus mykiss Walbaum) after co-culturing with live probiotics. HK leucocytes and gut cells adjusted to 5 × 106 and 2 × 106 ml-1, respectively, in L-15 medium containing 25% decomplemented FCS and 300 mg l-1 l-glutamine were co-cultured with Carnobacterium maltaromaticum B26 and C. divergens B33 at an multiplicity of infection of 25 for 6 and 12 h. Quantitative real-time reverse transcriptase polymerase chain reaction using SYBR Green I was employed to determine the mRNA expression of studied genes. Although neither probiotic strains significantly induced mRNA of the cytokines in gut cells, expression ratios of IL-1ß and TNF-a of HK cells were significantly higher, suggesting that these bacteria can stimulate innate immunity in rainbow trout. © 2006 Elsevier B.V. All rights reserved.

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KW - Real-time PCR

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Cytokine expression in leucocytes and gut cells of rainbow trout, Oncorhynchus mykiss Walbaum, induced by probiotics

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Keywords

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