Abstract
The effects of cyclic AMP on mitogen-activated protein (MAP) kinase activation were investigated in 3T3-F442A preadipocytes. Several agents, which raise intracellular cyclic AMP levels by distinct mechanisms, induced a transient activation of the p42 and p44 isoforms of MAP kinase and of a MAP kinase kinase. Activation of MAP kinase by cyclic AMP was prevented by two distinct inhibitors of cyclic AMP-dependent protein kinase and by PD 098059, a specific inhibitor of the activation of the MAP kinase kinase MEK 1. Therefore, in contrast to most cell types studied, cyclic AMP exerts a positive influence on the MAP kinase pathway in 3T3-F442A preadipocytes at a level upstream of the activation of MAP kinase kinase.
Original language | English |
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Pages (from-to) | 734-739 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 224 |
Issue number | 3 |
DOIs | |
Publication status | Published - 25 Jul 1996 |
Keywords
- 3T3 Cells
- Adipocytes
- Animals
- Calcium-Calmodulin-Dependent Protein Kinases
- Cell Differentiation
- Colforsin
- Cyclic AMP
- Cyclic AMP-Dependent Protein Kinases
- Enzyme Activation
- Enzyme Inhibitors
- Epidermal Growth Factor
- Flavonoids
- Mice
- Mitogen-Activated Protein Kinase 1
- Mitogen-Activated Protein Kinase 3
- Mitogen-Activated Protein Kinases
- Phosphorylation
- Protein-Tyrosine Kinases