Cyclic AMP stimulates the phosphorylation and activation of p42 and p44 mitogen-activated protein kinases in 3T3-F442A preadipocytes

S. J. Yarwood, E. Kilgour, N. G. Anderson

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    The effects of cyclic AMP on mitogen-activated protein (MAP) kinase activation were investigated in 3T3-F442A preadipocytes. Several agents, which raise intracellular cyclic AMP levels by distinct mechanisms, induced a transient activation of the p42 and p44 isoforms of MAP kinase and of a MAP kinase kinase. Activation of MAP kinase by cyclic AMP was prevented by two distinct inhibitors of cyclic AMP-dependent protein kinase and by PD 098059, a specific inhibitor of the activation of the MAP kinase kinase MEK 1. Therefore, in contrast to most cell types studied, cyclic AMP exerts a positive influence on the MAP kinase pathway in 3T3-F442A preadipocytes at a level upstream of the activation of MAP kinase kinase.

    Original languageEnglish
    Pages (from-to)734-739
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume224
    Issue number3
    DOIs
    Publication statusPublished - 25 Jul 1996

    Keywords

    • 3T3 Cells
    • Adipocytes
    • Animals
    • Calcium-Calmodulin-Dependent Protein Kinases
    • Cell Differentiation
    • Colforsin
    • Cyclic AMP
    • Cyclic AMP-Dependent Protein Kinases
    • Enzyme Activation
    • Enzyme Inhibitors
    • Epidermal Growth Factor
    • Flavonoids
    • Mice
    • Mitogen-Activated Protein Kinase 1
    • Mitogen-Activated Protein Kinase 3
    • Mitogen-Activated Protein Kinases
    • Phosphorylation
    • Protein-Tyrosine Kinases

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