Cultivating aerobic hydrocarbon-degrading bacteria from micro-algae

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

Abstract

Over the past three decades, we have dramatically increased our knowledge on the types of bacteria playing key roles in the degradation of oil hydrocarbons in the ocean. However, there remains a lack of complete understanding regarding the full diversity of species contributing a role in hydrocarbon degradative processes. Recent evidence highlights the cell surface, or ‘phycosphere’, of micro-algae as a new and largely underexplored biotope in the ocean that harbours novel taxa of hydrocarbon-degrading bacteria, including obligate hydrocarbonoclastic bacteria (OHCB). OHCB are a ‘specialised’ group exhibiting an almost exclusive requirement for hydrocarbon substrates as a sole source of carbon and energy. Several novel species of OHCB, including one new family, were recently discovered from laboratory cultures of marine micro-algae and more likely await discovery. The protocol below provides a detailed description for isolating these types of organisms from marine micro-algae based on their ability to degrade volatile low-molecular-weight hydrocarbons, aliphatic hydrocarbons, and/or polycyclic aromatic hydrocarbons.
Original languageEnglish
Title of host publicationHydrocarbon and Lipid Microbiology Protocols, Springer Protocols Handbooks
EditorsT. McGenity
PublisherSpringer
DOIs
Publication statusPublished - Nov 2014

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    Gutierrez, T. (2014). Cultivating aerobic hydrocarbon-degrading bacteria from micro-algae. In T. McGenity (Ed.), Hydrocarbon and Lipid Microbiology Protocols, Springer Protocols Handbooks [DOI 10.1007/8623_2014_1] Springer. https://doi.org/10.1007/8623_2014_1