Crystallization and preliminary x-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina

Sara L Pealing; Dominikus A Lysek; Paul Taylor; Dmitry Alexeev; Graeme A Reid; Stephen K Chapman; Malcolm D Walkinshaw

doi:10.1006/jsbi.1999.4139

Crystallization and preliminary x-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina

Sara L Pealing, Dominikus A Lysek, Paul Taylor, Dmitry Alexeev, Graeme A Reid, Stephen K Chapman, Malcolm D Walkinshaw

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

The fumarate reductase (flavocytochrome cs) from Shewanella frigidimarina (formerly S. putrefaciens) NCIMB400 has been crystallized in the space group P2(1), with cell dimensions of a = 45.447 Angstrom, b = 92.107 Angstrom, c = 78.311 Angstrom, and beta = 91.038 degrees and one molecule per asymmetric unit. A native data set has been collected to 1.8 Angstrom. The gene encoding Fcc(3) from the S. frigidimarina type strain ACAM591 has been cloned and sequenced and the protein crystallized in space group P21 with cell dimensions of a = 45.359 Angstrom, b = 88.051 Angstrom, c = 77.473 Angstrom, and beta = 104.499 degrees. Anomalous data have also been collected from the NCIMB400 crystal allowing the heme iron positions to be identified. (C) 1999 Academic Press.

Original language	English
Pages (from-to)	76-78
Number of pages	3
Journal	Journal of Structural Biology
Volume	127
Issue number	1
DOIs	https://doi.org/10.1006/jsbi.1999.4139
Publication status	Published - Aug 1999

Keywords

fumarate reductase
PUTREFACIENS
x-ray diffraction
Shewanella frigidimarina
flavocytochrome c(3)

Access to Document

10.1006/jsbi.1999.4139

Cite this

@article{6f1fb337015748f3b48546abf248137c,

title = "Crystallization and preliminary x-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina",

abstract = "The fumarate reductase (flavocytochrome cs) from Shewanella frigidimarina (formerly S. putrefaciens) NCIMB400 has been crystallized in the space group P2(1), with cell dimensions of a = 45.447 Angstrom, b = 92.107 Angstrom, c = 78.311 Angstrom, and beta = 91.038 degrees and one molecule per asymmetric unit. A native data set has been collected to 1.8 Angstrom. The gene encoding Fcc(3) from the S. frigidimarina type strain ACAM591 has been cloned and sequenced and the protein crystallized in space group P21 with cell dimensions of a = 45.359 Angstrom, b = 88.051 Angstrom, c = 77.473 Angstrom, and beta = 104.499 degrees. Anomalous data have also been collected from the NCIMB400 crystal allowing the heme iron positions to be identified. (C) 1999 Academic Press.",

keywords = "fumarate reductase, PUTREFACIENS, x-ray diffraction, Shewanella frigidimarina, flavocytochrome c(3)",

author = "Pealing, {Sara L} and Lysek, {Dominikus A} and Paul Taylor and Dmitry Alexeev and Reid, {Graeme A} and Chapman, {Stephen K} and Walkinshaw, {Malcolm D}",

note = "M1 - Article",

year = "1999",

month = aug,

doi = "10.1006/jsbi.1999.4139",

language = "English",

volume = "127",

pages = "76--78",

journal = "Journal of Structural Biology",

issn = "1047-8477",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Crystallization and preliminary x-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina

AU - Pealing, Sara L

AU - Lysek, Dominikus A

AU - Taylor, Paul

AU - Alexeev, Dmitry

AU - Reid, Graeme A

AU - Chapman, Stephen K

AU - Walkinshaw, Malcolm D

N1 - M1 - Article

PY - 1999/8

Y1 - 1999/8

N2 - The fumarate reductase (flavocytochrome cs) from Shewanella frigidimarina (formerly S. putrefaciens) NCIMB400 has been crystallized in the space group P2(1), with cell dimensions of a = 45.447 Angstrom, b = 92.107 Angstrom, c = 78.311 Angstrom, and beta = 91.038 degrees and one molecule per asymmetric unit. A native data set has been collected to 1.8 Angstrom. The gene encoding Fcc(3) from the S. frigidimarina type strain ACAM591 has been cloned and sequenced and the protein crystallized in space group P21 with cell dimensions of a = 45.359 Angstrom, b = 88.051 Angstrom, c = 77.473 Angstrom, and beta = 104.499 degrees. Anomalous data have also been collected from the NCIMB400 crystal allowing the heme iron positions to be identified. (C) 1999 Academic Press.

AB - The fumarate reductase (flavocytochrome cs) from Shewanella frigidimarina (formerly S. putrefaciens) NCIMB400 has been crystallized in the space group P2(1), with cell dimensions of a = 45.447 Angstrom, b = 92.107 Angstrom, c = 78.311 Angstrom, and beta = 91.038 degrees and one molecule per asymmetric unit. A native data set has been collected to 1.8 Angstrom. The gene encoding Fcc(3) from the S. frigidimarina type strain ACAM591 has been cloned and sequenced and the protein crystallized in space group P21 with cell dimensions of a = 45.359 Angstrom, b = 88.051 Angstrom, c = 77.473 Angstrom, and beta = 104.499 degrees. Anomalous data have also been collected from the NCIMB400 crystal allowing the heme iron positions to be identified. (C) 1999 Academic Press.

KW - fumarate reductase

KW - PUTREFACIENS

KW - x-ray diffraction

KW - Shewanella frigidimarina

KW - flavocytochrome c(3)

U2 - 10.1006/jsbi.1999.4139

DO - 10.1006/jsbi.1999.4139

M3 - Article

C2 - 10479620

SN - 1047-8477

VL - 127

SP - 76

EP - 78

JO - Journal of Structural Biology

JF - Journal of Structural Biology

IS - 1

ER -

Crystallization and preliminary x-ray analysis of flavocytochrome c(3), the fumarate reductase from Shewanella frigidimarina

Abstract

Keywords

Access to Document

Fingerprint

Cite this