Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus

Magda El-Bendary, Fergus G. Priest, J. F. Charles, Wilfrid J. Mitchell

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Insertional mutations in the spo0A and spoIIAC genes of Bacillus sphaericus 2362 were prepared by conjugation with Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS-PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalFEMS Microbiology Letters
Volume252
Issue number1
DOIs
Publication statusPublished - 1 Nov 2005

Keywords

  • Asporogenic mutants
  • Bacillus sphaericus
  • Insecticidal toxin

Fingerprint Dive into the research topics of 'Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus'. Together they form a unique fingerprint.

  • Cite this