Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus

Magda El-Bendary; Fergus G. Priest; J. F. Charles; Wilfrid J. Mitchell

doi:10.1016/j.femsle.2005.08.027

Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus

Magda El-Bendary, Fergus G. Priest, J. F. Charles, Wilfrid J. Mitchell

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Insertional mutations in the spo0A and spoIIAC genes of Bacillus sphaericus 2362 were prepared by conjugation with Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS-PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	51-56
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	252
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2005.08.027
Publication status	Published - 1 Nov 2005

Keywords

Asporogenic mutants
Bacillus sphaericus
Insecticidal toxin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.femsle.2005.08.027

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abstract = "Insertional mutations in the spo0A and spoIIAC genes of Bacillus sphaericus 2362 were prepared by conjugation with Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS-PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes. {\textcopyright} 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.",

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T1 - Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus

AU - El-Bendary, Magda

AU - Priest, Fergus G.

AU - Charles, J. F.

AU - Mitchell, Wilfrid J.

PY - 2005/11/1

Y1 - 2005/11/1

N2 - Insertional mutations in the spo0A and spoIIAC genes of Bacillus sphaericus 2362 were prepared by conjugation with Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS-PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

AB - Insertional mutations in the spo0A and spoIIAC genes of Bacillus sphaericus 2362 were prepared by conjugation with Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS-PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

KW - Asporogenic mutants

KW - Bacillus sphaericus

KW - Insecticidal toxin

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DO - 10.1016/j.femsle.2005.08.027

M3 - Article

SN - 0378-1097

VL - 252

SP - 51

EP - 56

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus

Abstract

Keywords

UN SDGs

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