Crystal protein synthesis is dependent on early sporulation gene expression in Bacillus sphaericus

Magda El-Bendary, Fergus G. Priest, J. F. Charles, Wilfrid J. Mitchell

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    27 Citations (Scopus)

    Abstract

    Insertional mutations in the spo0A and spoIIAC genes of Bacillus sphaericus 2362 were prepared by conjugation with Escherichia coli using a suicide plasmid containing cloned portions of the target genes. The mutants resembled their Bacillus subtilis counterparts phenotypically and were devoid of crystal proteins as determined by electron microscopy, SDS-PAGE and Western blots. The mutants had greatly reduced toxicity to anopheline mosquito larvae compared to the parental strain. We conclude that crystal protein synthesis in this bacterium is dependent on expression of early sporulation genes. © 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)51-56
    Number of pages6
    JournalFEMS Microbiology Letters
    Volume252
    Issue number1
    DOIs
    Publication statusPublished - 1 Nov 2005

    Keywords

    • Asporogenic mutants
    • Bacillus sphaericus
    • Insecticidal toxin

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