Cross-platform transcriptomic profiling of the response to recombinant human erythropoietin

Guan Wang*, Traci Kitaoka, Ali Crawford, Qian Mao, Andrew Hesketh, Fergus M. Guppy, Garrett I. Ash, Jason Liu, Mark B. Gerstein, Yannis P. Pitsiladis

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

RNA-seq has matured and become an important tool for studying RNA biology. Here we compared two RNA-seq (MGI DNBSEQ and Illumina NextSeq 500) and two microarray platforms (GeneChip Human Transcriptome Array 2.0 and Illumina Expression BeadChip) in healthy individuals administered recombinant human erythropoietin for transcriptome-wide quantification of differential gene expression. The results show that total RNA DNB-seq generated a multitude of target genes compared to other platforms. Pathway enrichment analyses revealed genes correlate to not only erythropoiesis and oxygen transport but also a wide range of other functions, such as tissue protection and immune regulation. This study provides a knowledge base of genes relevant to EPO biology through cross-platform comparisons and validation.

Original languageEnglish
Article number21705
JournalScientific Reports
Volume11
DOIs
Publication statusPublished - 4 Nov 2021

ASJC Scopus subject areas

  • General

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