Control of cell morphology of the yeast Saccharomyces cerevisiae by nutrient limitation in continuous culture

H. Kuriyama, John Colin Slaughter

    Research output: Contribution to journalArticle

    Abstract

    Saccharomyces cerevisiae IFO 0203, a polyploid yeast used in ethanol production in Japan, grows as ovoid cells in unstirred batch culture and on fully nutritive agar plates (2% w/v glucose; 0.67% w/v Difco yeast nitrogen base). Extensively branched pseudohyphae formed on 0.01% w/v ammonium sulphate plates within a few days. In continuous culture with high oxygen supply and limiting glucose, cells were elongated but growth was vigorous and the daughter cells separated well after budding. Limitation of growth by either nitrogen source or oxygen during continuous culture resulted in formation of truncated, occasionally branched, pseudohyphae up to five cells in length.

    Original languageEnglish
    Pages (from-to)37-40
    Number of pages4
    JournalLetters in Applied Microbiology
    Volume20
    Issue number1
    Publication statusPublished - 1995

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