Concentration of large volumes of water for waterborne pathogen detection using a tangential flow filtration based system

Marc Phillipe Yves Desmulliez, Abdelfateh Kerrouche, Stefan Wilhem, Helen Bridle

Research output: Contribution to conferencePoster

Abstract

Waterborne pathogens are a major concern in both developing and developed countries for the supply of safe drinking water used for consumption or food preparation. The concentration of the pathogens in water is extremely low. So, sample preparation is an important stage which is required for the detection of pathogens; as large volumes of sample need to be processed.

A sample processing device for medium scale manufacturing has been developed. The system concentrates and enriches water containing Cryptosporidium parvum and Giardia lamblia (oo)cysts from a large volume of water in order to enable further detection processes.
The device module concentrates a large sample volume (e.g. 50L, 500L) into a small volume (around 580 mL) while retaining the biological species. This system is based on a tangent hollow fibre filter (module E 1m2 150kD PAN) manufactured by NBS Biological. Fluid and particles smaller than the filter pore size (800nm) escape the circulation to a drain connection until the desired amount of input water is processed. However, the system batch processes only 90L in an hour, exceeding the 1000L in 24 hours recommended by the US EPA (Environmental Protection Agency) 1623 method.


Controlled experiments have been performed using fluorescent particles (latex beads) with diameters of 1μm and 5μm provided by Cospheric. A high recovery rate of 70% was confirmed.
Original languageEnglish
Publication statusPublished - 2015

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