Computer-aided multichannel detection in high-performance liquid chromatography

Anthony F Fell, Hugh P Scott, R. Gill, A. C. Moffat

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)


The development of a computer-aided rapid-scanning detector for HPLC based on the linear photodiode array UV-visible spectrophotometer is described. Chomatograms monitored at up to three wavelengths, with automatic capture of spectra for eluted peaks and post-run processing of spectral data to generate log10 (A) spectra, second derivative and fourth derivative spectra, are described. Methods are reported for the analysis of forensic samples of diacetylmorphine (heroin) in the presence of the degradation products and potential contaminants caffeine, papaverine, 6-acetylcodeine, thebaine, 6-acetyl-morphine, procaine and morphine separated by HPLC. The novel use of second and higher derivative spectra for the qualitative characterisation of drugs and contaminants separated by HPLC is described. © 1982 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

Original languageEnglish
Pages (from-to)69-78
Number of pages10
Issue number1
Publication statusPublished - Dec 1982


  • Diacetylmorphine
  • Forensic analysis
  • High-performance liquid chromatography
  • Higher derivative spectroscopy
  • Rapid-scanning multichannel detection


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