Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster

S. Langkilde, M. Schroder, T. Frank, L.V.T. Shepherd, S. Conner, H.V. Davies, O. Meyer, J. Danier, M. Rychlik, W.R. Belknap, K.F. McCue, K-H. Engel, D. Stewart, I Knudsen, M. Poulsen

    Research output: Contribution to journalArticle

    Abstract

    Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs a-chaconine and a-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of a-solanine: a-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced a-solanine content, and the parental control line (Desirée wild-type) having a traditional a-solanine: a-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60% freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different a-solanine: a-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.
    Copyright © 2012 Elsevier Inc. All rights reserved.
    Original languageEnglish
    Pages (from-to)177-185
    Number of pages9
    JournalRegulatory Toxicology and Pharmacology
    Volume64
    Issue number1
    DOIs
    Publication statusPublished - Oct 2012

    Fingerprint

    Solanine
    Mesocricetus
    Solanum tuberosum
    Toxicology
    Solanaceae
    Safety
    Nutritive Value
    Powders
    Diet

    Keywords

    • Glycoalkaloid
    • α-Solanine
    • α-Chaconine
    • GM
    • potato
    • Acetylcholinesterase (AChE)
    • hamster
    • toxicity
    • compositional analyses

    Cite this

    Langkilde, S. ; Schroder, M. ; Frank, T. ; Shepherd, L.V.T. ; Conner, S. ; Davies, H.V. ; Meyer, O. ; Danier, J. ; Rychlik, M. ; Belknap, W.R. ; McCue, K.F. ; Engel, K-H. ; Stewart, D. ; Knudsen, I ; Poulsen, M. / Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster. In: Regulatory Toxicology and Pharmacology . 2012 ; Vol. 64, No. 1. pp. 177-185.
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    title = "Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster",
    abstract = "Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs a-chaconine and a-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of a-solanine: a-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced a-solanine content, and the parental control line (Desir{\'e}e wild-type) having a traditional a-solanine: a-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60{\%} freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different a-solanine: a-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.Copyright {\circledC} 2012 Elsevier Inc. All rights reserved.",
    keywords = "Glycoalkaloid, α-Solanine, α-Chaconine, GM, potato, Acetylcholinesterase (AChE), hamster, toxicity, compositional analyses",
    author = "S. Langkilde and M. Schroder and T. Frank and L.V.T. Shepherd and S. Conner and H.V. Davies and O. Meyer and J. Danier and M. Rychlik and W.R. Belknap and K.F. McCue and K-H. Engel and D. Stewart and I Knudsen and M. Poulsen",
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    Langkilde, S, Schroder, M, Frank, T, Shepherd, LVT, Conner, S, Davies, HV, Meyer, O, Danier, J, Rychlik, M, Belknap, WR, McCue, KF, Engel, K-H, Stewart, D, Knudsen, I & Poulsen, M 2012, 'Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster', Regulatory Toxicology and Pharmacology , vol. 64, no. 1, pp. 177-185. https://doi.org/10.1016/j.yrtph.2012.06.018

    Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster. / Langkilde, S.; Schroder, M.; Frank, T.; Shepherd, L.V.T.; Conner, S.; Davies, H.V.; Meyer, O.; Danier, J.; Rychlik, M.; Belknap, W.R.; McCue, K.F.; Engel, K-H.; Stewart, D.; Knudsen, I; Poulsen, M.

    In: Regulatory Toxicology and Pharmacology , Vol. 64, No. 1, 10.2012, p. 177-185.

    Research output: Contribution to journalArticle

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    AU - Schroder, M.

    AU - Frank, T.

    AU - Shepherd, L.V.T.

    AU - Conner, S.

    AU - Davies, H.V.

    AU - Meyer, O.

    AU - Danier, J.

    AU - Rychlik, M.

    AU - Belknap, W.R.

    AU - McCue, K.F.

    AU - Engel, K-H.

    AU - Stewart, D.

    AU - Knudsen, I

    AU - Poulsen, M.

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    N2 - Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs a-chaconine and a-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of a-solanine: a-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced a-solanine content, and the parental control line (Desirée wild-type) having a traditional a-solanine: a-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60% freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different a-solanine: a-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.Copyright © 2012 Elsevier Inc. All rights reserved.

    AB - Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs a-chaconine and a-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of a-solanine: a-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced a-solanine content, and the parental control line (Desirée wild-type) having a traditional a-solanine: a-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60% freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different a-solanine: a-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.Copyright © 2012 Elsevier Inc. All rights reserved.

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    KW - Acetylcholinesterase (AChE)

    KW - hamster

    KW - toxicity

    KW - compositional analyses

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