Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster

S. Langkilde; M. Schroder; T. Frank; L.V.T. Shepherd; S. Conner; H.V. Davies; O. Meyer; J. Danier; M. Rychlik; W.R. Belknap; K.F. McCue; K-H. Engel; D. Stewart; I Knudsen; M. Poulsen

doi:10.1016/j.yrtph.2012.06.018

Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster

S. Langkilde, M. Schroder, T. Frank, L.V.T. Shepherd, S. Conner, H.V. Davies, O. Meyer, J. Danier, M. Rychlik, W.R. Belknap, K.F. McCue, K-H. Engel, D. Stewart, I Knudsen, M. Poulsen

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Abstract

Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs a-chaconine and a-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of a-solanine: a-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced a-solanine content, and the parental control line (Desirée wild-type) having a traditional a-solanine: a-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60% freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different a-solanine: a-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.
Copyright © 2012 Elsevier Inc. All rights reserved.

Original language	English
Pages (from-to)	177-185
Number of pages	9
Journal	Regulatory Toxicology and Pharmacology
Volume	64
Issue number	1
DOIs	https://doi.org/10.1016/j.yrtph.2012.06.018
Publication status	Published - Oct 2012

Keywords

Glycoalkaloid
α-Solanine
α-Chaconine
GM
potato
Acetylcholinesterase (AChE)
hamster
toxicity
compositional analyses

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10.1016/j.yrtph.2012.06.018

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Langkilde, S., Schroder, M., Frank, T., Shepherd, L. V. T., Conner, S., Davies, H. V., Meyer, O., Danier, J., Rychlik, M., Belknap, W. R., McCue, K. F., Engel, K.-H., Stewart, D., Knudsen, I., & Poulsen, M. (2012). Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster. Regulatory Toxicology and Pharmacology , 64(1), 177-185. https://doi.org/10.1016/j.yrtph.2012.06.018

@article{d2643576a1664212b044cd1bd1758494,

title = "Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster",

abstract = "Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs a-chaconine and a-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of a-solanine: a-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced a-solanine content, and the parental control line (Desir{\'e}e wild-type) having a traditional a-solanine: a-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60% freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different a-solanine: a-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.Copyright {\textcopyright} 2012 Elsevier Inc. All rights reserved.",

keywords = "Glycoalkaloid, α-Solanine, α-Chaconine, GM, potato, Acetylcholinesterase (AChE), hamster, toxicity, compositional analyses",

author = "S. Langkilde and M. Schroder and T. Frank and L.V.T. Shepherd and S. Conner and H.V. Davies and O. Meyer and J. Danier and M. Rychlik and W.R. Belknap and K.F. McCue and K-H. Engel and D. Stewart and I Knudsen and M. Poulsen",

year = "2012",

month = oct,

doi = "10.1016/j.yrtph.2012.06.018",

language = "English",

volume = "64",

pages = "177--185",

journal = "Regulatory Toxicology and Pharmacology ",

issn = "0273-2300",

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number = "1",

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Langkilde, S, Schroder, M, Frank, T, Shepherd, LVT, Conner, S, Davies, HV, Meyer, O, Danier, J, Rychlik, M, Belknap, WR, McCue, KF, Engel, K-H, Stewart, D, Knudsen, I & Poulsen, M 2012, 'Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster', Regulatory Toxicology and Pharmacology , vol. 64, no. 1, pp. 177-185. https://doi.org/10.1016/j.yrtph.2012.06.018

TY - JOUR

T1 - Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster

AU - Langkilde, S.

AU - Schroder, M.

AU - Frank, T.

AU - Shepherd, L.V.T.

AU - Conner, S.

AU - Davies, H.V.

AU - Meyer, O.

AU - Danier, J.

AU - Rychlik, M.

AU - Belknap, W.R.

AU - McCue, K.F.

AU - Engel, K-H.

AU - Stewart, D.

AU - Knudsen, I

AU - Poulsen, M.

PY - 2012/10

Y1 - 2012/10

N2 - Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs a-chaconine and a-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of a-solanine: a-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced a-solanine content, and the parental control line (Desirée wild-type) having a traditional a-solanine: a-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60% freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different a-solanine: a-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.Copyright © 2012 Elsevier Inc. All rights reserved.

AB - Steroidal glycoalkaloids (GAs) are toxins, produced by plants of the Solanaceae family. The potato plant (Solanum tuberosum L.) and its tubers predominantly contain the two GAs a-chaconine and a-solanine. These compounds are believed to act in synergy, and the degree of toxicity may therefore depend on their ratio in the potato. To determine the influence of a-solanine: a-chaconine ratio in potatoes on toxicity, a GM potato line (SGT 9-2) with reduced a-solanine content, and the parental control line (Desirée wild-type) having a traditional a-solanine: a-chaconine ratio were (1) studied for compositional similarity by analysing for a range of potato constituents, and (2) used in a 90-day feeding trial with the Syrian Golden hamster to study differential toxicity. The animal feeding study used diets with up to 60% freeze-dried potato powder from either line. Whilst data indicated some compositional differences between the GM line and its wildtype control these did not raise concerns related to nutritional value or safety. Results of the feeding trials showed a low number of significant differences between potato lines with different a-solanine: a-chaconine ratio but none were considered to raise safety concerns with regard to human (or animal) consumption.Copyright © 2012 Elsevier Inc. All rights reserved.

KW - Glycoalkaloid

KW - α-Solanine

KW - α-Chaconine

KW - GM

KW - potato

KW - Acetylcholinesterase (AChE)

KW - hamster

KW - toxicity

KW - compositional analyses

U2 - 10.1016/j.yrtph.2012.06.018

DO - 10.1016/j.yrtph.2012.06.018

M3 - Article

C2 - 22796474

SN - 0273-2300

VL - 64

SP - 177

EP - 185

JO - Regulatory Toxicology and Pharmacology

JF - Regulatory Toxicology and Pharmacology

IS - 1

ER -

Compositional and toxicological analysis of a GM potato line with reduced α-solanine content - a 90-day feeding study in the Syrian Golden hamster

Abstract

Keywords

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