Comparative molecular analysis of ovine and bovine Streptococcus uberis isolates

T. L. Gilchrist, David George Emslie Smith, J. L. Fitzpatrick, R. N. Zadoks, M. C. Fontaine

    Research output: Contribution to journalArticle

    Abstract

    Streptococcus uberis causes clinical and subclinical mastitis in cattle and sheep, but it is unknown whether the composition of Strep. uberis populations differs between host species. To address this, we characterized a collection of bovine and ovine Strep. uberis isolates with shared geographical and temporal origins by means of an expanded multilocus sequence typing scheme. Among 14 ovine and 35 bovine isolates, 35 allelic profiles were detected. Each allelic profile was associated with a single host species and all but one were new to the multilocus sequence typing database. The median number of new alleles per isolate was higher for ovine isolates than for bovine isolates. None of the ovine isolates belonged to the global clonal complexes 5 or 143, which are commonly associated with bovine mastitis and which have a wide geographical distribution. Ovine isolates also differed from bovine isolates in carriage of plasminogen activator genes, with significantly higher prevalence of pauB in ovine isolates. Isolates that were negative for yqiL, one of the targets of multilocus sequence typing, were found among ovine and bovine isolates and were not associated with a specific sequence type or global clonal complex. One bovine isolate carried a gapC allele that was probably acquired through lateral gene transfer, most likely from Streptococcus salivarius. We conclude that ovine isolates are distinct from bovine isolates of Strep. uberis, and that recombination between isolates from different host species or bacterial species could contribute to changes in virulence gene profiles with relevance for vaccine development.

    Original languageEnglish
    Pages (from-to)962-970
    Number of pages9
    JournalJournal of Dairy Science
    Volume96
    Issue number2
    DOIs
    Publication statusPublished - Feb 2013

    Fingerprint

    Streptococcus
    Sheep
    Multilocus Sequence Typing
    Alleles
    Bovine Mastitis
    Horizontal Gene Transfer
    Mastitis
    Plasminogen Activators
    Genetic Recombination
    Genes
    Virulence
    Vaccines
    Databases
    Population

    Keywords

    • Animals
    • Cattle
    • DNA, Bacterial
    • Female
    • Mastitis
    • Mastitis, Bovine
    • Multilocus Sequence Typing
    • Polymerase Chain Reaction
    • Sequence Analysis, DNA
    • Sheep
    • Sheep Diseases
    • Streptococcal Infections
    • Streptococcus

    Cite this

    Gilchrist, T. L. ; Smith, David George Emslie ; Fitzpatrick, J. L. ; Zadoks, R. N. ; Fontaine, M. C. / Comparative molecular analysis of ovine and bovine Streptococcus uberis isolates. In: Journal of Dairy Science. 2013 ; Vol. 96, No. 2. pp. 962-970.
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    Comparative molecular analysis of ovine and bovine Streptococcus uberis isolates. / Gilchrist, T. L.; Smith, David George Emslie; Fitzpatrick, J. L.; Zadoks, R. N.; Fontaine, M. C.

    In: Journal of Dairy Science, Vol. 96, No. 2, 02.2013, p. 962-970.

    Research output: Contribution to journalArticle

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    T1 - Comparative molecular analysis of ovine and bovine Streptococcus uberis isolates

    AU - Gilchrist, T. L.

    AU - Smith, David George Emslie

    AU - Fitzpatrick, J. L.

    AU - Zadoks, R. N.

    AU - Fontaine, M. C.

    N1 - Copyright © 2013 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

    PY - 2013/2

    Y1 - 2013/2

    N2 - Streptococcus uberis causes clinical and subclinical mastitis in cattle and sheep, but it is unknown whether the composition of Strep. uberis populations differs between host species. To address this, we characterized a collection of bovine and ovine Strep. uberis isolates with shared geographical and temporal origins by means of an expanded multilocus sequence typing scheme. Among 14 ovine and 35 bovine isolates, 35 allelic profiles were detected. Each allelic profile was associated with a single host species and all but one were new to the multilocus sequence typing database. The median number of new alleles per isolate was higher for ovine isolates than for bovine isolates. None of the ovine isolates belonged to the global clonal complexes 5 or 143, which are commonly associated with bovine mastitis and which have a wide geographical distribution. Ovine isolates also differed from bovine isolates in carriage of plasminogen activator genes, with significantly higher prevalence of pauB in ovine isolates. Isolates that were negative for yqiL, one of the targets of multilocus sequence typing, were found among ovine and bovine isolates and were not associated with a specific sequence type or global clonal complex. One bovine isolate carried a gapC allele that was probably acquired through lateral gene transfer, most likely from Streptococcus salivarius. We conclude that ovine isolates are distinct from bovine isolates of Strep. uberis, and that recombination between isolates from different host species or bacterial species could contribute to changes in virulence gene profiles with relevance for vaccine development.

    AB - Streptococcus uberis causes clinical and subclinical mastitis in cattle and sheep, but it is unknown whether the composition of Strep. uberis populations differs between host species. To address this, we characterized a collection of bovine and ovine Strep. uberis isolates with shared geographical and temporal origins by means of an expanded multilocus sequence typing scheme. Among 14 ovine and 35 bovine isolates, 35 allelic profiles were detected. Each allelic profile was associated with a single host species and all but one were new to the multilocus sequence typing database. The median number of new alleles per isolate was higher for ovine isolates than for bovine isolates. None of the ovine isolates belonged to the global clonal complexes 5 or 143, which are commonly associated with bovine mastitis and which have a wide geographical distribution. Ovine isolates also differed from bovine isolates in carriage of plasminogen activator genes, with significantly higher prevalence of pauB in ovine isolates. Isolates that were negative for yqiL, one of the targets of multilocus sequence typing, were found among ovine and bovine isolates and were not associated with a specific sequence type or global clonal complex. One bovine isolate carried a gapC allele that was probably acquired through lateral gene transfer, most likely from Streptococcus salivarius. We conclude that ovine isolates are distinct from bovine isolates of Strep. uberis, and that recombination between isolates from different host species or bacterial species could contribute to changes in virulence gene profiles with relevance for vaccine development.

    KW - Animals

    KW - Cattle

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    KW - Female

    KW - Mastitis

    KW - Mastitis, Bovine

    KW - Multilocus Sequence Typing

    KW - Polymerase Chain Reaction

    KW - Sequence Analysis, DNA

    KW - Sheep

    KW - Sheep Diseases

    KW - Streptococcal Infections

    KW - Streptococcus

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    DO - 10.3168/jds.2012-5705

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