Comparative evaluation of methods for assessing peak homogeneity in liquid isocratic reversed-phase chromatography

T. P. Bridge, Howard Williams, A. F. Fell

Research output: Contribution to journalArticle

Abstract

A significant problem in the development of an automated system for the interpretation of chromatographic profiles is the detection of cases of co-elution without the use of a considerable amount of computer time or intervention by a human operator. A partial solution to this problem is offered by a range of tests which can be implemented on a suitable microcomputer to provide an estimate of peak homogeneity. Several such tests are described and their application to the three-dimensional data sets produced by a photodiode-array detector is illustrated. The sensitivity of each of the different tests to the presence of a second component varies according to the relative amplitude, separation and spectral similarity of the two components. The extent of this variation is assessed for each of the tests and an indication is given of the reliability of each method under typical chromatographic conditions. © 1989.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalAnalytica Chimica Acta
Volume223
Issue numberC
Publication statusPublished - 1989

Fingerprint Dive into the research topics of 'Comparative evaluation of methods for assessing peak homogeneity in liquid isocratic reversed-phase chromatography'. Together they form a unique fingerprint.

Cite this