Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus

Preben Nielsen; Frederick A. Rainey; Helle Outtrup; Fergus G. Priest; Dagmar Fritze

doi:10.1016/0378-1097(94)90171-6

Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus

Preben Nielsen, Frederick A. Rainey, Helle Outtrup, Fergus G. Priest, Dagmar Fritze

Research output: Contribution to journal › Article › peer-review

118 Citations (Scopus)

Abstract

Comparative sequence analysis of the 16S rDNA of 14 alkaliphilic or alkalitolerant, Gram-positive, aerobic, endo-spore forming bacterial strains was performed. Bacillus alcalophilus DSM 485(T) and Bacillus cohnii DSM 6307(T) were included to represent the two validly described alkaliphiles assigned to the genus Bacillus. The majority of isolates (8 strains) clustered with B. alcalophilus DSM 485(T) forming a distinct phylogenetic group (rRNA group 6) within the radiation of the genus Bacillus and related taxa. Bacillus cohnii DSM 6307(T) and two of the isolates, DSM 8719 and DSM 8723, grouped with B. fastidiosus and B. megaterium and are allocated to rRNA group 1. The remaining two strains DSM 8720 and DSM 8721 show an equidistant relationship to both groups.

Original language	English
Pages (from-to)	61-65
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	117
Issue number	1
DOIs	https://doi.org/10.1016/0378-1097(94)90171-6
Publication status	Published - 1994

Keywords

16s rRNA (rDNA)
Alkaliphilic
Bacillus
Phylogeny
rRNA group 6

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10.1016/0378-1097(94)90171-6

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title = "Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus",

abstract = "Comparative sequence analysis of the 16S rDNA of 14 alkaliphilic or alkalitolerant, Gram-positive, aerobic, endo-spore forming bacterial strains was performed. Bacillus alcalophilus DSM 485(T) and Bacillus cohnii DSM 6307(T) were included to represent the two validly described alkaliphiles assigned to the genus Bacillus. The majority of isolates (8 strains) clustered with B. alcalophilus DSM 485(T) forming a distinct phylogenetic group (rRNA group 6) within the radiation of the genus Bacillus and related taxa. Bacillus cohnii DSM 6307(T) and two of the isolates, DSM 8719 and DSM 8723, grouped with B. fastidiosus and B. megaterium and are allocated to rRNA group 1. The remaining two strains DSM 8720 and DSM 8721 show an equidistant relationship to both groups.",

keywords = "16s rRNA (rDNA), Alkaliphilic, Bacillus, Phylogeny, rRNA group 6",

author = "Preben Nielsen and Rainey, {Frederick A.} and Helle Outtrup and Priest, {Fergus G.} and Dagmar Fritze",

year = "1994",

doi = "10.1016/0378-1097(94)90171-6",

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T1 - Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus

AU - Nielsen, Preben

AU - Rainey, Frederick A.

AU - Outtrup, Helle

AU - Priest, Fergus G.

AU - Fritze, Dagmar

PY - 1994

Y1 - 1994

N2 - Comparative sequence analysis of the 16S rDNA of 14 alkaliphilic or alkalitolerant, Gram-positive, aerobic, endo-spore forming bacterial strains was performed. Bacillus alcalophilus DSM 485(T) and Bacillus cohnii DSM 6307(T) were included to represent the two validly described alkaliphiles assigned to the genus Bacillus. The majority of isolates (8 strains) clustered with B. alcalophilus DSM 485(T) forming a distinct phylogenetic group (rRNA group 6) within the radiation of the genus Bacillus and related taxa. Bacillus cohnii DSM 6307(T) and two of the isolates, DSM 8719 and DSM 8723, grouped with B. fastidiosus and B. megaterium and are allocated to rRNA group 1. The remaining two strains DSM 8720 and DSM 8721 show an equidistant relationship to both groups.

AB - Comparative sequence analysis of the 16S rDNA of 14 alkaliphilic or alkalitolerant, Gram-positive, aerobic, endo-spore forming bacterial strains was performed. Bacillus alcalophilus DSM 485(T) and Bacillus cohnii DSM 6307(T) were included to represent the two validly described alkaliphiles assigned to the genus Bacillus. The majority of isolates (8 strains) clustered with B. alcalophilus DSM 485(T) forming a distinct phylogenetic group (rRNA group 6) within the radiation of the genus Bacillus and related taxa. Bacillus cohnii DSM 6307(T) and two of the isolates, DSM 8719 and DSM 8723, grouped with B. fastidiosus and B. megaterium and are allocated to rRNA group 1. The remaining two strains DSM 8720 and DSM 8721 show an equidistant relationship to both groups.

KW - 16s rRNA (rDNA)

KW - Alkaliphilic

KW - Bacillus

KW - Phylogeny

KW - rRNA group 6

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DO - 10.1016/0378-1097(94)90171-6

M3 - Article

SN - 0378-1097

VL - 117

SP - 61

EP - 65

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

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ER -

Comparative 16S rDNA sequence analysis of some alkaliphilic bacilli and the establishment of a sixth rRNA group within the genus Bacillus

Abstract

Keywords

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