Combined fluorescence lifetime and surface topographical imaging of biological tissue

Charlotte Hopkinson; Andrew B. Matheson; Neil Finlayson; Michael G. Tanner; Ahsan R. Akram; Robert K. Henderson

doi:10.1364/BOE.504309

Combined fluorescence lifetime and surface topographical imaging of biological tissue

Charlotte Hopkinson, Andrew B. Matheson, Neil Finlayson, Michael G. Tanner, Ahsan R. Akram, Robert K. Henderson

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Abstract

In this work a combined fluorescence lifetime and surface topographical imaging system is demonstrated. Based around a 126 × 192 time resolved single photon avalanche diode (SPAD) array operating in time correlated single-photon counting (TCSPC) mode, both the fluorescence lifetime and time of flight (ToF) can be calculated on a pixel by pixel basis. Initial tests on fluorescent samples show it is able to provide 4 mm resolution in distance and 0.4 ns resolution in lifetime. This combined modality has potential biomedical applications such as surgical guidance, endoscopy, and diagnostic imaging. The system is demonstrated on both ovine and human pulmonary tissue samples, where it offers excellent fluorescence lifetime contrast whilst also giving a measure of the distance to the sample surface.

Original language	English
Pages (from-to)	212-221
Number of pages	10
Journal	Biomedical Optics Express
Volume	15
Issue number	1
Early online date	14 Dec 2023
DOIs	https://doi.org/10.1364/BOE.504309
Publication status	Published - 1 Jan 2024

Keywords

Atomic and Molecular Physics, and Optics
Biotechnology

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Biotechnology

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10.1364/BOE.504309Licence: CC BY

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title = "Combined fluorescence lifetime and surface topographical imaging of biological tissue",

abstract = "In this work a combined fluorescence lifetime and surface topographical imaging system is demonstrated. Based around a 126 × 192 time resolved single photon avalanche diode (SPAD) array operating in time correlated single-photon counting (TCSPC) mode, both the fluorescence lifetime and time of flight (ToF) can be calculated on a pixel by pixel basis. Initial tests on fluorescent samples show it is able to provide 4 mm resolution in distance and 0.4 ns resolution in lifetime. This combined modality has potential biomedical applications such as surgical guidance, endoscopy, and diagnostic imaging. The system is demonstrated on both ovine and human pulmonary tissue samples, where it offers excellent fluorescence lifetime contrast whilst also giving a measure of the distance to the sample surface.",

keywords = "Atomic and Molecular Physics, and Optics, Biotechnology",

author = "Charlotte Hopkinson and Matheson, {Andrew B.} and Neil Finlayson and Tanner, {Michael G.} and Akram, {Ahsan R.} and Henderson, {Robert K.}",

note = "Funding Information: Engineering and Physical Sciences Research Council (EP/R005257/1). The authors would like to thank Horiba (Glasgow) for kindly providing the FLIMera TCSPC camera used in this work, and Dr. Graham Hungerford in particular for providing guidance on its operation. The authors are also grateful to the staff of the Thoracic Surgery and Pathology departments at the Royal Infirmary of Edinburgh for facilitating tissue samples. Publisher Copyright: {\textcopyright} 2024 OSA - The Optical Society. All rights reserved.",

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doi = "10.1364/BOE.504309",

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AU - Hopkinson, Charlotte

AU - Matheson, Andrew B.

AU - Finlayson, Neil

AU - Tanner, Michael G.

AU - Akram, Ahsan R.

AU - Henderson, Robert K.

N1 - Funding Information: Engineering and Physical Sciences Research Council (EP/R005257/1). The authors would like to thank Horiba (Glasgow) for kindly providing the FLIMera TCSPC camera used in this work, and Dr. Graham Hungerford in particular for providing guidance on its operation. The authors are also grateful to the staff of the Thoracic Surgery and Pathology departments at the Royal Infirmary of Edinburgh for facilitating tissue samples. Publisher Copyright: © 2024 OSA - The Optical Society. All rights reserved.

PY - 2024/1/1

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N2 - In this work a combined fluorescence lifetime and surface topographical imaging system is demonstrated. Based around a 126 × 192 time resolved single photon avalanche diode (SPAD) array operating in time correlated single-photon counting (TCSPC) mode, both the fluorescence lifetime and time of flight (ToF) can be calculated on a pixel by pixel basis. Initial tests on fluorescent samples show it is able to provide 4 mm resolution in distance and 0.4 ns resolution in lifetime. This combined modality has potential biomedical applications such as surgical guidance, endoscopy, and diagnostic imaging. The system is demonstrated on both ovine and human pulmonary tissue samples, where it offers excellent fluorescence lifetime contrast whilst also giving a measure of the distance to the sample surface.

AB - In this work a combined fluorescence lifetime and surface topographical imaging system is demonstrated. Based around a 126 × 192 time resolved single photon avalanche diode (SPAD) array operating in time correlated single-photon counting (TCSPC) mode, both the fluorescence lifetime and time of flight (ToF) can be calculated on a pixel by pixel basis. Initial tests on fluorescent samples show it is able to provide 4 mm resolution in distance and 0.4 ns resolution in lifetime. This combined modality has potential biomedical applications such as surgical guidance, endoscopy, and diagnostic imaging. The system is demonstrated on both ovine and human pulmonary tissue samples, where it offers excellent fluorescence lifetime contrast whilst also giving a measure of the distance to the sample surface.

KW - Atomic and Molecular Physics, and Optics

KW - Biotechnology

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JO - Biomedical Optics Express

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Combined fluorescence lifetime and surface topographical imaging of biological tissue

Abstract

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