Combined fluorescence lifetime and surface topographical imaging of biological tissue

Charlotte Hopkinson, Andrew B. Matheson, Neil Finlayson, Michael G. Tanner, Ahsan R. Akram, Robert K. Henderson

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Abstract

In this work a combined fluorescence lifetime and surface topographical imaging system is demonstrated. Based around a 126 × 192 time resolved single photon avalanche diode (SPAD) array operating in time correlated single-photon counting (TCSPC) mode, both the fluorescence lifetime and time of flight (ToF) can be calculated on a pixel by pixel basis. Initial tests on fluorescent samples show it is able to provide 4 mm resolution in distance and 0.4 ns resolution in lifetime. This combined modality has potential biomedical applications such as surgical guidance, endoscopy, and diagnostic imaging. The system is demonstrated on both ovine and human pulmonary tissue samples, where it offers excellent fluorescence lifetime contrast whilst also giving a measure of the distance to the sample surface.
Original languageEnglish
Pages (from-to)212-221
Number of pages10
JournalBiomedical Optics Express
Volume15
Issue number1
Early online date14 Dec 2023
DOIs
Publication statusPublished - 1 Jan 2024

Keywords

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Biotechnology

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