Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique

P. L. Jørgensen; Martin Tangney; Poul Erik Pedersen; Sven Hastrup; Børge Diderichsen; Steen T. Jørgensen

doi:10.1128/AEM.66.2.825-827.2000

Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique

P. L. Jørgensen, Martin Tangney, Poul Erik Pedersen, Sven Hastrup, Børge Diderichsen, Steen T. Jørgensen

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

A gene encoding an alkaline protease was cloned from an alkalophilic bacillus, and its nucleotide sequence was determined. The cloned gene was used to increase the copy number of the protease gene on the chromosome by an improved gene amplification technique.

Original language	English
Pages (from-to)	825-827
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	66
Issue number	2
DOIs	https://doi.org/10.1128/AEM.66.2.825-827.2000
Publication status	Published - Feb 2000

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10.1128/AEM.66.2.825-827.2000

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title = "Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique",

abstract = "A gene encoding an alkaline protease was cloned from an alkalophilic bacillus, and its nucleotide sequence was determined. The cloned gene was used to increase the copy number of the protease gene on the chromosome by an improved gene amplification technique.",

author = "J{\o}rgensen, {P. L.} and Martin Tangney and Pedersen, {Poul Erik} and Sven Hastrup and B{\o}rge Diderichsen and J{\o}rgensen, {Steen T.}",

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Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique. / Jørgensen, P. L.; Tangney, Martin; Pedersen, Poul Erik et al.

In: Applied and Environmental Microbiology, Vol. 66, No. 2, 02.2000, p. 825-827.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique

AU - Jørgensen, P. L.

AU - Tangney, Martin

AU - Pedersen, Poul Erik

AU - Hastrup, Sven

AU - Diderichsen, Børge

AU - Jørgensen, Steen T.

PY - 2000/2

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N2 - A gene encoding an alkaline protease was cloned from an alkalophilic bacillus, and its nucleotide sequence was determined. The cloned gene was used to increase the copy number of the protease gene on the chromosome by an improved gene amplification technique.

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U2 - 10.1128/AEM.66.2.825-827.2000

DO - 10.1128/AEM.66.2.825-827.2000

M3 - Article

SN - 0099-2240

VL - 66

SP - 825

EP - 827

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 2

ER -

Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique

Abstract

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