Cloning and sequencing of an alkaline protease gene from Bacillus lentus and amplification of the gene on the B. lentus chromosome by an improved technique

P. L. Jørgensen, Martin Tangney, Poul Erik Pedersen, Sven Hastrup, Børge Diderichsen, Steen T. Jørgensen

    Research output: Contribution to journalArticlepeer-review

    21 Citations (Scopus)

    Abstract

    A gene encoding an alkaline protease was cloned from an alkalophilic bacillus, and its nucleotide sequence was determined. The cloned gene was used to increase the copy number of the protease gene on the chromosome by an improved gene amplification technique.

    Original languageEnglish
    Pages (from-to)825-827
    Number of pages3
    JournalApplied and Environmental Microbiology
    Volume66
    Issue number2
    DOIs
    Publication statusPublished - Feb 2000

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