Cloning and nucleotide sequence of a thermostable cyclodextrin glycosyltransferase gene from Thermoanaerobacter sp. ATCC 53627 and its expression in Escherichia coli

S. T. Jørgensen, M. Tangney, R. L. Starnes, K. Amemiya, P. L. Jørgensen

    Research output: Contribution to journalArticle

    20 Citations (Scopus)

    Abstract

    A gene, cgtA, encoding an extremely thermostable cyclodextrin glycosyltransferase (CGTase) was cloned from a thermophilic anaerobe, Thermoanaerobacter sp. ATCC 53627, and expressed in Escherichia coli. DNA and protein sequencing revealed that the mature enzyme of 683 amino acid residues (MW 75 kDa) was preceded by a signal peptide of 27 amino acid residues. The sequence of the Thermoanaerobacter CGTase was similar to sequences of Bacillus CGTases, with more than 58% identity, and very similar (89% identity) to a CGTase enzyme from Thermoanaerobacterium thermosulfurogenes.

    Original languageEnglish
    Pages (from-to)1027-1031
    Number of pages5
    JournalBiotechnology Letters
    Volume19
    Issue number10
    DOIs
    Publication statusPublished - 1997

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