TY - JOUR
T1 - Cloning and nucleotide sequence of a thermostable cyclodextrin glycosyltransferase gene from Thermoanaerobacter sp. ATCC 53627 and its expression in Escherichia coli
AU - Jørgensen, S. T.
AU - Tangney, M.
AU - Starnes, R. L.
AU - Amemiya, K.
AU - Jørgensen, P. L.
PY - 1997
Y1 - 1997
N2 - A gene, cgtA, encoding an extremely thermostable cyclodextrin glycosyltransferase (CGTase) was cloned from a thermophilic anaerobe, Thermoanaerobacter sp. ATCC 53627, and expressed in Escherichia coli. DNA and protein sequencing revealed that the mature enzyme of 683 amino acid residues (MW 75 kDa) was preceded by a signal peptide of 27 amino acid residues. The sequence of the Thermoanaerobacter CGTase was similar to sequences of Bacillus CGTases, with more than 58% identity, and very similar (89% identity) to a CGTase enzyme from Thermoanaerobacterium thermosulfurogenes.
AB - A gene, cgtA, encoding an extremely thermostable cyclodextrin glycosyltransferase (CGTase) was cloned from a thermophilic anaerobe, Thermoanaerobacter sp. ATCC 53627, and expressed in Escherichia coli. DNA and protein sequencing revealed that the mature enzyme of 683 amino acid residues (MW 75 kDa) was preceded by a signal peptide of 27 amino acid residues. The sequence of the Thermoanaerobacter CGTase was similar to sequences of Bacillus CGTases, with more than 58% identity, and very similar (89% identity) to a CGTase enzyme from Thermoanaerobacterium thermosulfurogenes.
UR - http://www.scopus.com/inward/record.url?scp=0030662111&partnerID=8YFLogxK
U2 - 10.1023/A:1018459703742
DO - 10.1023/A:1018459703742
M3 - Article
SN - 0141-5492
VL - 19
SP - 1027
EP - 1031
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 10
ER -