TY - JOUR
T1 - Circulating angiogenic cell response to sprint interval and continuous exercise
AU - O’Carroll, Louis
AU - Wardrop, Bruce
AU - Murphy, Ronan P.
AU - Ross, Mark D.
AU - Harrison, Michael
N1 - Funding Information:
Acknowledgements This study was supported by Technological Sector Research Strand I funding to Waterford Institute of Technology.
Publisher Copyright:
© 2019, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2019/3/6
Y1 - 2019/3/6
N2 - Introduction: Although commonly understood as immune cells, certain T lymphocyte and monocyte subsets have angiogenic potential, contributing to blood vessel growth and repair. These cells are highly exercise responsive and may contribute to the cardiovascular benefits seen with exercise.Purpose: To compare the effects of a single bout of continuous (CONTEX) and sprint interval exercise (SPRINT) on circulating angiogenic cells (CAC) in healthy recreationally active adults.Methods: Twelve participants (aged 29 ± 2 years, BMI 25.5 ± 0.9 kg m−2 , V˙O2 peak 44.3 ± 1.8 ml kg−1 min−1; mean ± SEM) participated in the study. Participants completed a 45-min bout of CONTEX at 70% peak oxygen uptake and 6 × 20 s sprints on a cycle ergometer, in a counterbalanced design. Blood was sampled pre-, post-, 2 h and 24 h post-exercise for quantification of CAC subsets by whole blood flow cytometric analysis. Angiogenic T lymphocytes (TANG) and angiogenic Tie2-expressing monocytes (TEM) were identified by the expression of CD31 and Tie2, respectively.Results: Circulating (cells µL−1) CD3+CD31+ TANG increased immediately post-exercise in both trials (p < 0.05), with a significantly greater increase (p < 0.05) following SPRINT (+ 57%) compared to CONTEX (+ 14%). Exercise increased (p < 0.05) the expression of the chemokine receptor CXCR4 on TANG at 24 h. Tie2-expressing classical (CD14++CD16−), intermediate (CD14++CD16+) and non-classical (CD14+CD16++) monocytes and circulating CD34+ CD45dim progenitor cells were higher post-exercise in SPRINT, but unchanged in CONTEX. All post-exercise increases in SPRINT were back to pre-exercise levels at 2 h and 24 h.Conclusion: Acute exercise transiently increases circulating TANG, TEM and progenitor cells with greater increases evident following very high intensity sprint exercise than following prolonged continuous paced endurance exercise.
AB - Introduction: Although commonly understood as immune cells, certain T lymphocyte and monocyte subsets have angiogenic potential, contributing to blood vessel growth and repair. These cells are highly exercise responsive and may contribute to the cardiovascular benefits seen with exercise.Purpose: To compare the effects of a single bout of continuous (CONTEX) and sprint interval exercise (SPRINT) on circulating angiogenic cells (CAC) in healthy recreationally active adults.Methods: Twelve participants (aged 29 ± 2 years, BMI 25.5 ± 0.9 kg m−2 , V˙O2 peak 44.3 ± 1.8 ml kg−1 min−1; mean ± SEM) participated in the study. Participants completed a 45-min bout of CONTEX at 70% peak oxygen uptake and 6 × 20 s sprints on a cycle ergometer, in a counterbalanced design. Blood was sampled pre-, post-, 2 h and 24 h post-exercise for quantification of CAC subsets by whole blood flow cytometric analysis. Angiogenic T lymphocytes (TANG) and angiogenic Tie2-expressing monocytes (TEM) were identified by the expression of CD31 and Tie2, respectively.Results: Circulating (cells µL−1) CD3+CD31+ TANG increased immediately post-exercise in both trials (p < 0.05), with a significantly greater increase (p < 0.05) following SPRINT (+ 57%) compared to CONTEX (+ 14%). Exercise increased (p < 0.05) the expression of the chemokine receptor CXCR4 on TANG at 24 h. Tie2-expressing classical (CD14++CD16−), intermediate (CD14++CD16+) and non-classical (CD14+CD16++) monocytes and circulating CD34+ CD45dim progenitor cells were higher post-exercise in SPRINT, but unchanged in CONTEX. All post-exercise increases in SPRINT were back to pre-exercise levels at 2 h and 24 h.Conclusion: Acute exercise transiently increases circulating TANG, TEM and progenitor cells with greater increases evident following very high intensity sprint exercise than following prolonged continuous paced endurance exercise.
KW - Angiogenic T cells
KW - Endothelial progenitor cells
KW - High intensity exercise
KW - Tie2 expressing monocytes
UR - http://www.scopus.com/inward/record.url?scp=85060599540&partnerID=8YFLogxK
U2 - 10.1007/s00421-018-04065-7
DO - 10.1007/s00421-018-04065-7
M3 - Article
C2 - 30673849
AN - SCOPUS:85060599540
SN - 1439-6319
VL - 119
SP - 743
EP - 752
JO - European Journal of Applied Physiology
JF - European Journal of Applied Physiology
IS - 3
ER -